The Catholic League of France, sometimes referred to by contemporary Catholics as the Holy League, was a major participant in the French Wars of Religion. The League, founded and led by Henry I, Duke of Guise, intended the eradication of Protestantism from Catholic France, as well as the replacement of the French King Henry III, who had acquiesced to Protestant worship in the Edict of Beaulieu (1576). The League also fought against Henry of Navarre, the Protestant prince who became presumptive heir to the French throne in 1584.

The French Wars of Religion were a series of civil wars between French Catholics and Protestants from 1562 to 1598. Between two and four million people died from violence, famine or disease directly caused by the conflict, and it severely damaged the power of the French monarchy. One of its most notorious episodes was the St. Bartholomew's Day massacre in 1572. The fighting ended with a compromise in 1598, when Henry of Navarre, who had converted to Catholicism in 1593, was proclaimed King Henry IV of France and issued the Edict of Nantes, which granted substantial rights and freedoms to the Huguenots. However, Catholics continued to disapprove of Protestants and of Henry, and his assassination in 1610 triggered a fresh round of Huguenot rebellions in the 1620s.