Germacradienol synthase

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Germacradienol synthase
Identifiers
EC number 4.2.3.22
CAS number 211049-88-6
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Germacradienol synthase (EC 4.2.3.22, germacradienol/germacrene-D synthase, 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase [(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol-forming]) is an enzyme with systematic name (2E,6E)-farnesyl-diphosphate diphosphate-lyase ((1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol-forming).[1][2][3] This enzyme catalyses the following chemical reaction

(2E,6E)-farnesyl diphosphate + H2O (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate

This enzyme requires Mg2+ for activity.

References[edit]

  1. ^ Cane, D.E.; Watt, R.M. (2003). "Expression and mechanistic analysis of a germacradienol synthase from Streptomyces coelicolor implicated in geosmin biosynthesis". Proc. Natl. Acad. Sci. USA. 100: 1547–1551. doi:10.1073/pnas.0337625100. PMC 149869Freely accessible. PMID 12556563. 
  2. ^ He, X.; Cane, D.E. (2004). "Mechanism and stereochemistry of the germacradienol/germacrene D synthase of Streptomyces coelicolor A3(2)". J. Am. Chem. Soc. 126: 2678–2679. doi:10.1021/ja039929k. PMID 14995166. 
  3. ^ Gust, B.; Challis, G.L.; Fowler, K.; Kieser, T.; Chater, K.F. (2003). "PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin". Proc. Natl. Acad. Sci. USA. 100 (4): 1541–1546. doi:10.1073/pnas.0337542100. PMC 149868Freely accessible. PMID 12563033. 

External links[edit]