Vetispiradiene synthase

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Vetispiradiene synthase
Identifiers
EC number 4.2.3.21
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

In enzymology, a vetispiradiene synthase (EC 4.2.3.21) is an enzyme of Egyptian henbane that catalyzes the following chemical reaction:

trans,trans-farnesyl diphosphate vetispiradiene + diphosphate

Hence, this enzyme has one substrate, trans,trans-farnesyl diphosphate, and two products, vetispiradiene and diphosphate.

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates, the systematic name of this enzyme class is trans,trans-farnesyl-diphosphate diphosphate-lyase (cyclizing, vetispiradiene-forming). Other names in common use include vetispiradiene-forming farnesyl pyrophosphate cyclase, pemnaspirodiene synthase, HVS, and vetispiradiene cyclase. This enzyme participates in terpenoid biosynthesis.

References[edit]

  • Back K, Chappell J (1995). "Cloning and bacterial expression of a sesquiterpene cyclase from Hyoscyamus muticus and its molecular comparison to related terpene cyclases". J. Biol. Chem. 270 (13): 7375–81. doi:10.1074/jbc.270.13.7375. PMID 7706281. 
  • Chappell J, Ricci P, Marco Y (1998). "Sesquiterpene cyclase is not a determining factor for elicitor- and pathogen-induced capsidiol accumulation in tobacco". Planta. 205 (3): 467–476. doi:10.1007/s004250050345. 
  • Mathis JR, Back K, Starks C, Noel J, Poulter CD, Chappell J (1997). "Pre-steady-state study of recombinant sesquiterpene cyclases". Biochemistry. 36 (27): 8340–8. doi:10.1021/bi963019g. PMID 9204881. 
  • Yoshioka H, Yamada N, Doke N (1999). "cDNA cloning of sesquiterpene cyclase and squalene synthase, and expression of the genes in potato tuber infected with Phytophthora infestans". Plant Cell Physiol. 40 (9): 993–8. PMID 10588069. 
  • Martin VJ, Yoshikuni Y, Keasling JD (2001). "The in vivo synthesis of plant sesquiterpenes by Escherichia coli". Biotechnol. Bioeng. 75 (5): 497–503. doi:10.1002/bit.10037. PMID 11745124.