4-Hydroxy-3-methylbut-2-enyl diphosphate reductase

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4-Hydroxy-3-methylbut-2-enyl diphosphate reductase
EC number
CAS number 512789-14-9
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

4-Hydroxy-3-methylbut-2-enyl diphosphate reductase (EC, isopentenyl-diphosphate:NADP+ oxidoreductase, LytB, (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase, HMBPP reductase, IspH, LytB/IspH) is an enzyme in the non-mevalonate pathway. It acts upon (E)-4-Hydroxy-3-methyl-but-2-enyl pyrophosphate (or "HMB-PP").[1][2][3][4][5][6]

(1) isopentenyl diphosphate + NAD(P)+ + H2O (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+
(2) dimethylallyl diphosphate + NAD(P)+ + H2O (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H + H+

4-Hydroxy-3-methylbut-2-enyl diphosphate reductase is an iron-sulfur protein that contains either a [3Fe-4S] or a [4Fe-4S] cluster.


  1. ^ Rohdich, F.; Hecht, S.; Glawischnig, K.; Adam, P.; Krieger, C.; Amslinger, S.; Arigoni, D.; Bacher, A.; Eisenreich, W. (2002). "Studies on the nonmevalonate terpene biosynthetic pathway: Metabolic role of IspH (LytB) protein". Proc. Natl. Acad. Sci. USA. 99: 1158–1163. doi:10.1073/pnas.032658999. PMC 122160Freely accessible. PMID 11818558. 
  2. ^ Hintz, M.; Reichenberg, A.; Altincicek, B.; Bahr, U.; Gschwind, R.M.; Kollas, A.-K.; Beck, E.; Wiesner, J.; Eberl, M.; Jomaa, H. (2001). "Identification of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate as a major activator for human T cells in Escherichia coli". FEBS Lett. 509: 317–322. doi:10.1016/s0014-5793(01)03191-x. PMID 11741609. 
  3. ^ Charon, L.; Pale-Grosdemange, C.; Rohmer, M. (1999). "On the reduction steps in the mevalonate independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis in the bacterium Zymomonas mobilis". Tetrahedron Lett. 40: 7231–7234. doi:10.1016/s0040-4039(99)01531-2. 
  4. ^ Röhrich, R.C.; Englert, N.; Troschke, K.; Reichenberg, A.; Hintz, M.; Seeber, F.; Balconi, E.; Aliverti, A.; Zanetti, G.; Köhler, U.; Pfeiffer, M.; Beck, E.; Jomaa, H.; Wiesner, J. (2005). "Reconstitution of an apicoplast-localised electron transfer pathway involved in the isoprenoid biosynthesis of Plasmodium falciparum". FEBS Lett. 579: 6433–6438. doi:10.1016/j.febslet.2005.10.037. PMID 16289098. 
  5. ^ Wolff, M.; Seemann, M.; Bui, T.S.B.; Frapart, Y.; Tritsch, D.; Garcia Estrabot, A.; Rodríguez-Concepción, M.; Boronat, A.; Marquet, A.; Rohmer, M. (2003). "Isoprenoid biosynthesis via the methylerythritol phosphate pathway: the (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (LytB/IspH) from Escherichia coli is a [4Fe-4S] protein". FEBS Lett. 541: 115–120. doi:10.1016/s0014-5793(03)00317-x. PMID 12706830. 
  6. ^ Gräwert, T.; Kaiser, J.; Zepeck, F.; Laupitz, R.; Hecht, S.; Amslinger, S.; Schramek, N.; Schleicher, E.; Weber, S.; Haslbeck, M.; Buchner, J.; Rieder, C.; Arigoni, D.; Bacher, A.; Eisenreich, W.; Rohdich, F. (2004). "IspH protein of Escherichia coli: studies on iron-sulfur cluster implementation and catalysis". J. Am. Chem. Soc. 126: 12847–12855. doi:10.1021/ja0471727. PMID 15469281.