Aliphatic (R)-hydroxynitrile lyase

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Aliphatic (R)-hydroxynitrile lyase
Identifiers
EC number 4.1.2.46
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Aliphatic (R)-hydroxynitrile lyase (EC 4.1.2.46, (R)-HNL, (R)-oxynitrilase, (R)-hydroxynitrile lyase, LuHNL) is an enzyme with systematic name (2R)-2-hydroxy-2-methylbutanenitrile butan-2-one-lyase (cyanide forming).[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction

(2R)-2-hydroxy-2-methylbutanenitrile cyanide + butan-2-one

The enzyme contains Zn2+.

References[edit]

  1. ^ Trummler, K.; Roos, J.; Schwaneberg, U.; Effenberger, F.; Förster, S.; Pfizenmaier, K.; Wajant, H. (1998). "Expression of the Zn2+-containing hydroxynitrile lyase from flax (Linum usitatissimum) in Pichia pastoris— utilization of the recombinant enzyme for enzymatic analysis and site-directed mutagenesis". Plant Sci. 139: 19–27. doi:10.1016/s0168-9452(98)00173-3. 
  2. ^ Trummler, K.; Wajant, H. (1997). "Molecular cloning of acetone cyanohydrin lyase from flax (Linum usitatissimum). Definition of a novel class of hydroxynitrile lyases". J. Biol. Chem. 272 (8): 4770–4774. doi:10.1074/jbc.272.8.4770. PMID 9030531. 
  3. ^ Albrecht J, Jansen I, Kula MR (1993). "Improved purification of an (R)-oxynitrilase from Linum usitatissimum (flax) and investigation of the substrate range". Biotechnol. Appl. Biochem. 17: 191–203. 
  4. ^ Xu, L.-L.; Singh, B.K.; Conn, E.E. (1988). "Purification and characterization of acetone cyanohydrin lyase from Linum usitatissimum". Arch. Biochem. Biophys. 263: 256–263. doi:10.1016/0003-9861(88)90634-0. PMID 3377504. 
  5. ^ Cabirol, F.L.; Tan, P.L.; Tay, B.; Cheng, S.; Hanefeld, U.; Sheldon, R.A. (2008). "Linum usitatissimum hydroxynitrile lyase cross-linked enzyme aggregates: a recyclable enantioselective catalyst". Adv. Synth. Catal. 350: 2329–2338. doi:10.1002/adsc.200800309. 
  6. ^ Breithaupt, H.; Pohl, M.; Bönigk, W.; Heim, P.; Schimz, K.-L.; Kula, M.-R. (1999). "Cloning and expression of (R)-hydroxynitrile lyase from Linum usitatissimum (flax)". J. Mol. Catal. B. 6: 315–332. doi:10.1016/S1381-1177(98)00109-X. 

External links[edit]