Diacetyl reductase ((S)-acetoin forming)

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Diacetyl reductase ((S)-acetoin forming)
Identifiers
EC number 1.1.1.304
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Diacetyl reductase ((S)-acetoin forming) (EC 1.1.1.304, (S)-acetoin dehydrogenase) is an enzyme with systematic name (S)-acetoin:NAD+ oxidoreductase.[1][2][3] This enzyme catalyses the following chemical reaction

(S)-acetoin + NAD+ diacetyl + NADH + H+

The reaction is catalysed in the reverse direction, this activity is usually associated with butanediol dehydrogenase activity (EC 1.1.1.4 or EC 1.1.1.76). While the butanediol dehydrogenase activity is reversible, diacetyl reductase activity is irreversible, this enzyme has been reported in the bacteria Geobacillus stearothermophilus, Enterobacter aerogenes and Klebsiella pneumoniae. Different from EC 1.1.1.303, diacetyl reductase ((R)-acetoin forming).

References[edit]

  1. ^ Giovannini, P.P.; Medici, A.; Bergamini, C.M.; Rippa, M. (1996). "Properties of diacetyl (acetoin) reductase from Bacillus stearothermophilus". Bioorg. Med. Chem. 4: 1197–1201. doi:10.1016/0968-0896(96)00086-7. PMID 8879540. 
  2. ^ Carballo, J.; Martin, R.; Bernardo, A.; Gonzalez, J. (1991). "Purification, characterization and some properties of diacetyl(acetoin) reductase from Enterobacter aerogenes". Eur. J. Biochem. 198: 327–332. doi:10.1111/j.1432-1033.1991.tb16019.x. PMID 2040298. 
  3. ^ Ui S, Okajima Y, Mimura A, Kanai H, Kobayashi T, Kudo T (1997). "Sequence analysis of the gene for and characterization of D-acetoin forming meso-2,3-butanediol dehydrogenase of Klebsiella pneumoniae expressed in Escherichia coli". J. Ferment. Bioeng. 83: 32–37. doi:10.1016/s0922-338x(97)87323-0.