(S)-canadine synthase

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(S)-canadine synthase
EC number
CAS number 114308-22-4
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / QuickGO

In enzymology, a (S)-canadine synthase (EC is an enzyme that catalyzes the chemical reaction

(S)-tetrahydrocolumbamine + NADPH + H+ + O2 (S)-canadine + NADP+ + 2 H2O

The 4 substrates of this enzyme are (S)-tetrahydrocolumbamine, NADPH, H+, and O2, whereas its 3 products are (S)-canadine, NADP+, and H2O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and the other dehydrogenated. The systematic name of this enzyme class is (S)-tetrahydrocolumbamine,NADPH:oxygen oxidoreductase (methylenedioxy-bridge-forming). Other names in common use include (S)-tetrahydroberberine synthase, and (S)-tetrahydrocolumbamine oxidase (methylenedioxy-bridge-forming). This enzyme participates in alkaloid biosynthesis i. It employs one cofactor, heme-thiolate(P-450).


  • Rueffer M; Zenk MH (1994). "Canadine synthase from Thalictrum tuberosum cell cultures catalyses the formation of the methylenedioxy bridge in berberine synthesis". Phytochemistry. 36 (5): 1219–1223. doi:10.1016/S0031-9422(00)89640-5.