(butirosin acyl-carrier protein)—L-glutamate ligase (EC 126.96.36.199, [BtrI acyl-carrier protein]—L-glutamate ligase, BtrJ) is an enzyme with systematic name (BtrI acyl-carrier protein):L-glutamate ligase (ADP-forming). This enzyme catalyses the following chemical reaction
- (1) ATP + L-glutamate + BtrI acyl-carrier protein ADP + phosphate + L-glutamyl-[BtrI acyl-carrier protein]
- (2) ATP + L-glutamate + 4-amino butanoyl-[BtrI acyl-carrier protein] ADP + phosphate + 4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein]
This enzyme catalyses two steps in the biosynthesis of the side chain of the aminoglycoside antibiotics of the butirosin family.
- ^ Li, Y.; Llewellyn, N.M.; Giri, R.; Huang, F.; Spencer, J.B. (2005). "Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway". Chem. Biol. 12 (6): 665–675. doi:10.1016/j.chembiol.2005.04.010. PMID 15975512.