Deoxyhypusine synthase

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Deoxyhypusine synthase
Identifiers
EC number 2.5.1.46
CAS number 127069-31-2
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Deoxyhypusine synthase (EC 2.5.1.46, spermidine:eIF5A-lysine 4-aminobutyltransferase (propane-1,3-diamine-forming)) is an enzyme with systematic name (eIF5A-precursor)-lysine:spermidine 4-aminobutyltransferase (propane-1,3-diamine-forming).[1][2][3][4][5][6][7][8][9] This enzyme catalyses the following chemical reaction

[eIF5A-precursor]-lysine + spermidine [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine (overall reaction)
(1a) spermidine + NAD+ dehydrospermidine + NADH
(1b) dehydrospermidine + [enzyme]-lysine N-(4-aminobutylidene)-[enzyme]-lysine + propane-1,3-diamine
(1c) N-(4-aminobutylidene)-[enzyme]-lysine + [eIF5A-precursor]-lysine N-(4-aminobutylidene)-[eIF5A-precursor]-lysine + [enzyme]-lysine
(1d) N-(4-aminobutylidene)-[eIF5A-precursor]-lysine + NADH + H+ [eIF5A-precursor]-deoxyhypusine + NAD+

The eukaryotic initiation factor eIF5A contains a hypusine residue that is essential for activity.

References[edit]

  1. ^ Wolff, E.C.; Park, M.H.; Folk, J.E. (1990). "Cleavage of spermidine as the first step in deoxyhypusine synthesis. The role of NAD+". J. Biol. Chem. 265 (11): 4793–4799. PMID 1690726. 
  2. ^ Wolff, E.C.; Folk, J.E.; Park, M.H. (1997). "Enzyme-substrate intermediate formation at lysine 329 of human deoxyhypusine synthase". J. Biol. Chem. 272 (25): 15865–15871. doi:10.1074/jbc.272.25.15865. PMID 9188485. 
  3. ^ Chen, K.Y.; Liu, A.Y.C. (1997). "Biochemistry and function of hypusine formation on eukaryotic initiation factor 5A". Biol. Signals. 6 (3): 105–109. doi:10.1159/000109115. PMID 9285092. 
  4. ^ Ober, D.; Hartmann, T. (1999). "Deoxyhypusine synthase from tobacco. cDNA isolation, characterization, and bacterial expression of an enzyme with extended substrate specificity". J. Biol. Chem. 274 (45): 32040–32047. doi:10.1074/jbc.274.45.32040. PMID 10542236. 
  5. ^ Ober, D.; Hartmann, T. (1999). "Homospermidine synthase, the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis, evolved from deoxyhypusine synthase". Proc. Natl. Acad. Sci. USA. 96 (26): 14777–14782. Bibcode:1999PNAS...9614777O. doi:10.1073/pnas.96.26.14777. PMC 24724Freely accessible. PMID 10611289. 
  6. ^ Wolff, E.C.; Park, M.H. (1999). "Identification of lysine350 of yeast deoxyhypusine synthase as the site of enzyme intermediate formation". Yeast. 15 (1): 43–50. doi:10.1002/(SICI)1097-0061(19990115)15:1<43::AID-YEA344>3.0.CO;2-K. PMID 10028184. 
  7. ^ Wolff, E.C.; Wolff, J.; Park, M.H. (2000). "Deoxyhypusine synthase generates and uses bound NADH in a transient hydride transfer mechanism". J. Biol. Chem. 275 (13): 9170–9177. doi:10.1074/jbc.275.13.9170. PMID 10734052. 
  8. ^ Joe, Y.A.; Wolff, E.C.; Park, M.H. (1995). "Cloning and expression of human deoxyhypusine synthase cDNA: structure-function studies with the recombinant enzyme and mutant proteins". J. Biol. Chem. 270 (38): 22386–22392. doi:10.1074/jbc.270.38.22386. PMID 7673224. 
  9. ^ Tao, Y.; Chen, K.Y. (1995). "Molecular cloning and functional expression of Neurospora deoxyhypusine synthase cDNA and identification of yeast deoxyhypusine synthase cDNA". J. Biol. Chem. 270 (41): 23984–23987. doi:10.1074/jbc.270.41.23984. PMID 7592594. 

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