(S)-2-haloacid dehalogenase

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(S)-2-haloacid dehalogenase
EC number3.8.1.2
CAS number37289-39-7
IntEnzIntEnz view
ExPASyNiceZyme view
MetaCycmetabolic pathway
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, a (S)-2-haloacid dehalogenase (EC is an enzyme that catalyzes the chemical reaction

(S)-2-haloacid + H2O (R)-2-hydroxyacid + halide

Thus, the two substrates of this enzyme are (S)-2-haloacid and H2O, whereas its two products are (R)-2-hydroxyacid and halide.

This enzyme belongs to the family of hydrolases, specifically those acting on halide bonds in carbon-halide compounds. The systematic name of this enzyme class is (S)-2-haloacid halidohydrolase. Other names in common use include 2-haloacid dehalogenase[ambiguous], 2-haloacid halidohydrolase [ambiguous][ambiguous], 2-haloalkanoic acid dehalogenase, 2-haloalkanoid acid halidohydrolase, 2-halocarboxylic acid dehalogenase II, DL-2-haloacid dehalogenase[ambiguous], L-2-haloacid dehalogenase, and L-DEX. This enzyme participates in gamma-hexachlorocyclohexane degradation and 1,2-dichloroethane degradation.

Structural studies[edit]

As of late 2007, 10 structures have been solved for this class of enzymes, with PDB accession codes 1AQ6, 1JUD, 1QH9, 1QQ5, 1QQ6, 1QQ7, 1ZRM, 1ZRN, 2NO4, and 2NO5.


  • Goldman P, Milne GW, Keister DB (1968). "Carbon-halogen bond cleavage. 3. Studies on bacterial halidohrolases". J. Biol. Chem. 243 (2): 428&ndash, 34. PMID 5635785.
  • Motosugi M, Esaki N, Soda K (1982). "Preparation and properties of 2-halo acid dehalogenase from Pseudomonas putida". Agric. Biol. Chem. 46 (3): 837&ndash, 838. doi:10.1271/bbb1961.46.837.
  • Klages U, Krauss S, Lingens F (1983). "2-Haloacid dehalogenase from a 4-chlorobenzoate-degrading Pseudomonas spec. CBS 3". Hoppe-Seyler's Z. Physiol. Chem. 364 (5): 529&ndash, 35. doi:10.1515/bchm2.1983.364.1.529. PMID 6873881.
  • Diez A, Prieto MI, Alvarez MJ, Bautista JM, Garrido J, Puyet A (1996). "Improved catalytic performance of a 2-haloacid dehalogenase from Azotobacter sp. by ion-exchange immobilisation". Biochem. Biophys. Res. Commun. 220 (3): 828&ndash, 33. doi:10.1006/bbrc.1996.0489. PMID 8607850.
  • Morsberger FM, Muller R, Otto MK, Lingens F, Kulbe KD (1991). "Purification and characterization of 2-halocarboxylic acid dehalogenase II from Pseudomonas spec. CBS 3". Biol. Chem. Hoppe-Seyler. 372 (10): 915&ndash, 22. doi:10.1515/bchm3.1991.372.2.915. PMID 1772590.
  • Kohler R, Brokamp A, Schwarze R, Reiting RH, Schmidt FR (1998). "Characteristics and DNA-sequence of a cryptic haloalkanoic acid dehalogenase from Agrobacterium tumefaciens RS5". Curr. Microbiol. 36 (2): 96&ndash, 101. doi:10.1007/s002849900286. PMID 9425247.
  • Motosugi K, Esaki N, Soda K (1982). "Bacterial assimilation of D- and L-2-chloropropionates and occurrence of a new dehalogenase". Arch. Microbiol. 131 (3): 179&ndash, 83. doi:10.1007/BF00405875. PMID 7103659.
  • Kurihara T, Esaki N, Soda K (2000). "Bacterial 2-haloacid dehalogenases: structures and reaction mechanisms". J. Mol. Catal., B Enzym. 10: 57&ndash, 65. doi:10.1016/S1381-1177(00)00108-9.
  • M, Tsunasawa S; Esaki N (1996). "Bacterial 2-haloacid dehalogenases: Structures and catalytic properties". Pure Appl. Chem. 68 (11): 2097&ndash, 2103. doi:10.1351/pac199668112097.