CCA tRNA nucleotidyltransferase

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CCA tRNA nucleotidyltransferase
Identifiers
EC number 2.7.7.72
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

CCA tRNA nucleotidyltransferase (EC 2.7.7.72, CCA-adding enzyme, tRNA adenylyltransferase, tRNA CCA-pyrophosphorylase, tRNA-nucleotidyltransferase, transfer-RNA nucleotidyltransferase, transfer ribonucleic acid nucleotidyl transferase, CTP(ATP):tRNA nucleotidyltransferase, transfer ribonucleate adenylyltransferase, transfer ribonucleate adenyltransferase, transfer RNA adenylyltransferase, transfer ribonucleate nucleotidyltransferase, ATP (CTP):tRNA nucleotidyltransferase, ribonucleic cytidylic cytidylic adenylic pyrophosphorylase, transfer ribonucleic adenylyl (cytidylyl) transferase, transfer ribonucleic-terminal trinucleotide nucleotidyltransferase, transfer ribonucleate cytidylyltransferase, ribonucleic cytidylyltransferase, -C-C-A pyrophosphorylase, ATP(CTP)-tRNA nucleotidyltransferase, tRNA adenylyl(cytidylyl)transferase, CTP:tRNA cytidylyltransferase) is an enzyme with systematic name CTP,CTP,ATP:tRNA cytidylyl,cytidylyl,adenylyltransferase.[1][2][3][4][5] This enzyme catalyses the following chemical reaction

a tRNA precursor + 2 CTP + ATP a tRNA with a 3' CCA end + 3 diphosphate (overall reaction)
(1a) a tRNA precursor + CTP a tRNA with a 3' cytidine end + diphosphate
(1b) a tRNA with a 3' cytidine + CTP a tRNA with a 3' CC end + diphosphate
(1c) a tRNA with a 3' CC end + ATP a tRNA with a 3' CCA end + diphosphate

The acylation of all tRNAs with an amino acid occurs at the terminal ribose of a 3' CCA sequence.

See also[edit]

References[edit]

  1. ^ Schofield, P.; Williams, K.R. (1977). "Purification and some properties of Escherichia coli tRNA nucleotidyltransferase". J. Biol. Chem. 252 (15): 5584–5588. PMID 328503. 
  2. ^ Shi, P.Y.; Maizels, N.; Weiner, A.M. (1998). "CCA addition by tRNA nucleotidyltransferase: polymerization without translocation". EMBO J. 17 (11): 3197–3206. doi:10.1093/emboj/17.11.3197. PMC 1170658Freely accessible. PMID 9606201. 
  3. ^ Augustin, M.A.; Reichert, A.S.; Betat, H.; Huber, R.; Morl, M.; Steegborn, C. (2003). "Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization". J. Mol. Biol. 328: 985–994. doi:10.1016/S0022-2836(03)00381-4. PMID 12729736. 
  4. ^ Yakunin, A.F.; Proudfoot, M.; Kuznetsova, E.; Savchenko, A.; Brown, G.; Arrowsmith, C.H.; Edwards, A.M. (2004). "The HD domain of the Escherichia coli tRNA nucleotidyltransferase has 2′,3′-cyclic phosphodiesterase, 2′-nucleotidase, and phosphatase activities". J. Biol. Chem. 279 (35): 36819–36827. doi:10.1074/jbc.M405120200. PMID 15210699. 
  5. ^ Hou, Y.M. (2010). "CCA addition to tRNA: implications for tRNA quality control". IUBMB Life. 62 (4): 251–260. doi:10.1002/iub.301. PMC 2848691Freely accessible. PMID 20101632. 

External links[edit]