CCA tRNA nucleotidyltransferase

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CCA tRNA nucleotidyltransferase
EC number
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

CCA tRNA nucleotidyltransferase (EC, CCA-adding enzyme, tRNA adenylyltransferase, tRNA CCA-pyrophosphorylase, tRNA-nucleotidyltransferase, transfer-RNA nucleotidyltransferase, transfer ribonucleic acid nucleotidyl transferase, CTP(ATP):tRNA nucleotidyltransferase, transfer ribonucleate adenylyltransferase, transfer ribonucleate adenyltransferase, transfer RNA adenylyltransferase, transfer ribonucleate nucleotidyltransferase, ATP (CTP):tRNA nucleotidyltransferase, ribonucleic cytidylic cytidylic adenylic pyrophosphorylase, transfer ribonucleic adenylyl (cytidylyl) transferase, transfer ribonucleic-terminal trinucleotide nucleotidyltransferase, transfer ribonucleate cytidylyltransferase, ribonucleic cytidylyltransferase, -C-C-A pyrophosphorylase, ATP(CTP)-tRNA nucleotidyltransferase, tRNA adenylyl(cytidylyl)transferase, CTP:tRNA cytidylyltransferase) is an enzyme with systematic name CTP,CTP,ATP:tRNA cytidylyl,cytidylyl,adenylyltransferase.[1][2][3][4][5] This enzyme catalyses the following chemical reaction

a tRNA precursor + 2 CTP + ATP a tRNA with a 3' CCA end + 3 diphosphate (overall reaction)
(1a) a tRNA precursor + CTP a tRNA with a 3' cytidine end + diphosphate
(1b) a tRNA with a 3' cytidine + CTP a tRNA with a 3' CC end + diphosphate
(1c) a tRNA with a 3' CC end + ATP a tRNA with a 3' CCA end + diphosphate

The acylation of all tRNAs with an amino acid occurs at the terminal ribose of a 3' CCA sequence.

See also[edit]


  1. ^ Schofield, P.; Williams, K.R. (1977). "Purification and some properties of Escherichia coli tRNA nucleotidyltransferase". J. Biol. Chem. 252 (15): 5584–5588. PMID 328503. 
  2. ^ Shi, P.Y.; Maizels, N.; Weiner, A.M. (1998). "CCA addition by tRNA nucleotidyltransferase: polymerization without translocation". EMBO J. 17 (11): 3197–3206. doi:10.1093/emboj/17.11.3197. PMC 1170658Freely accessible. PMID 9606201. 
  3. ^ Augustin, M.A.; Reichert, A.S.; Betat, H.; Huber, R.; Morl, M.; Steegborn, C. (2003). "Crystal structure of the human CCA-adding enzyme: insights into template-independent polymerization". J. Mol. Biol. 328: 985–994. doi:10.1016/S0022-2836(03)00381-4. PMID 12729736. 
  4. ^ Yakunin, A.F.; Proudfoot, M.; Kuznetsova, E.; Savchenko, A.; Brown, G.; Arrowsmith, C.H.; Edwards, A.M. (2004). "The HD domain of the Escherichia coli tRNA nucleotidyltransferase has 2′,3′-cyclic phosphodiesterase, 2′-nucleotidase, and phosphatase activities". J. Biol. Chem. 279 (35): 36819–36827. doi:10.1074/jbc.M405120200. PMID 15210699. 
  5. ^ Hou, Y.M. (2010). "CCA addition to tRNA: implications for tRNA quality control". IUBMB Life. 62 (4): 251–260. doi:10.1002/iub.301. PMC 2848691Freely accessible. PMID 20101632. 

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