16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase

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16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase
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16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase (EC, S-adenosylmethionine-6-N',N'-adenosyl (rRNA) dimethyltransferase, KsgA, ksgA methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:16S rRNA (adenine1518-N6/adenine1519-N6)-dimethyltransferase.[1][2][3][4][5][6][7][8] This enzyme catalyses the following chemical reaction

4 S-adenosyl-L-methionine + adenine1518/adenine1519 in 16S rRNA 4 Ribosomal RNA + N6-dimethyladenine1518/N6-dimethyladenine1519 in 16S rRNA

KsgA introduces the dimethylation of adenine1518 and adenine1519 in 16S rRNA. Strains lacking the methylase are resistant to kasugamycin [1].


  1. ^ Helser, T.L.; Davies, J.E.; Dahlberg, J.E. (1971). "Change in methylation of 16S ribosomal RNA associated with mutation to kasugamycin resistance in Escherichia coli". Nature New Biology. 233: 12–14. doi:10.1038/newbio233012a0. PMID 4329247. 
  2. ^ Helser, T.L.; Davies, J.E.; Dahlberg, J.E. (1972). "Mechanism of kasugamycin resistance in Escherichia coli". Nature New Biology. 235: 6–9. doi:10.1038/newbio235006a0. PMID 4336392. 
  3. ^ van Buul, C.P.; van Knippenberg, P.H. (1985). "Nucleotide sequence of the ksgA gene of Escherichia coli: comparison of methyltransferases effecting dimethylation of adenosine in ribosomal RNA". Gene. 38: 65–72. doi:10.1016/0378-1119(85)90204-5. PMID 3905517. 
  4. ^ Formenoy, L.J.; Cunningham, P.R.; Nurse, K.; Pleij, C.W.; Ofengand, J. (1994). "Methylation of the conserved A1518-A1519 in Escherichia coli 16S ribosomal RNA by the ksgA methyltransferase is influenced by methylations around the similarly conserved U1512.G1523 base pair in the 3′ terminal hairpin". Biochimie. 76: 1123–1128. doi:10.1016/0300-9084(94)90040-x. PMID 7538324. 
  5. ^ O'Farrell, H.C.; Scarsdale, J.N.; Rife, J.P. (2004). "Crystal structure of KsgA, a universally conserved rRNA adenine dimethyltransferase in Escherichia coli". J. Mol. Biol. 339: 337–353. doi:10.1016/j.jmb.2004.02.068. PMID 15136037. 
  6. ^ Poldermans, B.; Roza, L.; Van Knippenberg, P.H. (1979). "Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3′ end of 16 S ribosomal RNA of Escherichia coli. III. Purification and properties of the methylating enzyme and methylase-30 S interactions". J. Biol. Chem. 254: 9094–9100. PMID 383712. 
  7. ^ Demirci, H.; Belardinelli, R.; Seri, E.; Gregory, S.T.; Gualerzi, C.; Dahlberg, A.E.; Jogl, G. (2009). "Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5′-methylthioadenosine". J. Mol. Biol. 388: 271–282. doi:10.1016/j.jmb.2009.02.066. PMC 2679894Freely accessible. PMID 19285505. 
  8. ^ Tu, C.; Tropea, J.E.; Austin, B.P.; Court, D.L.; Waugh, D.S.; Ji, X. (2009). "Structural basis for binding of RNA and cofactor by a KsgA methyltransferase". Structure. 17: 374–385. doi:10.1016/j.str.2009.01.010. PMC 2672589Freely accessible. PMID 19278652. 

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