2,5-diketocamphane 1,2-monooxygenase

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2,5-diketocamphane 1,2-monooxygenase
EC number
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

2,5-diketocamphane 1,2-monooxygenase (EC, 2,5-diketocamphane lactonizing enzyme, ketolactonase I, 2,5-diketocamphane 1,2-monooxygenase oxygenating component, 2,5-DKCMO, camP (gene), camphor 1,2-monooxygenase, camphor ketolactonase I) is an enzyme with systematic name (+)-bornane-2,5-dione,NADH:oxygen oxidoreductase (1,2-lactonizing).[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction

(+)-bornane-2,5-dione + O2 + NADH + H+ (+)-5-oxo-1,2-campholide + NAD+ + H2O

2,5-diketocamphane 1,2-monooxygenase is a flavoprotein (FMN) which requires Fe2+.


  1. ^ Conrad, H.E.; DuBus, R.; Namtvedt, M.J.; Gunsalus, I.C. (1965). "Mixed function oxidation. II. Separation and properties of the enzymes catalyzing camphor lactonizaton". J. Biol. Chem. 240: 495–503. PMID 14253460. 
  2. ^ Yu, C.A.; Gunsalus, I.C. (1969). "Monoxygenases. VII. Camphor ketolactonase I and the role of three protein components". J. Biol. Chem. 244 (22): 6149–6152. PMID 4310834. 
  3. ^ Taylor, D.G.; Trudgill, P.W. (1986). "Camphor revisited: studies of 2,5-diketocamphane 1,2-monooxygenase from Pseudomonas putida ATCC 17453". J. Bacteriol. 165 (2): 489–497. PMC 214445Freely accessible. PMID 3944058. 
  4. ^ Selifonov, S.A. (1992). "Microbial oxidation of adamantanone by Pseudomonas putida carrying the camphor catabolic plasmid". Biochem. Biophys. Res. Commun. 186 (3): 1429–1436. doi:10.1016/s0006-291x(05)81566-9. PMID 1510672. 
  5. ^ Jones, K.H.; Smith, R.T.; Trudgill, P.W. (1993). "Diketocamphane enantiomer-specific ’Baeyer-Villiger’ monooxygenases from camphor-grown Pseudomonas putida ATCC 17453". J. Gen. Microbiol. 139 (4): 797–805. doi:10.1099/00221287-139-4-797. PMID 8515237. 
  6. ^ Kadow, M.; Sass, S.; Schmidt, M.; Bornscheuer, U.T. (2011). "Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007". AMB Express. 1 (1): #13–13. doi:10.1186/2191-0855-1-13. PMC 3222318Freely accessible. PMID 21906366. 

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