Aika R-16: Virgin Mission is a Japanese three-episode OVA series animated by Studio Fantasia. Developed as a prequel to the Agent Aika OVA series, it was released on the tenth anniversary of said series. It takes place ten years prior to the events of Agent Aika and follows the story of Aika's first mission when she is 16 years old. The series was followed by a three-episode OVA sequel called Aika Zero where she is 19 years old. As with its predecessor, both series are known for their abundance of fan service in the form of panty shots and nudity. Aika R-16: Virgin Mission was acquired by Bandai Entertainment.

Ami Koshimizu is a Japanese actress and singer. She has voiced many different types of characters, from young girls to adult women, as well as boys and animals. Her roles include leads such as Ryuko Matoi in Kill la Kill, Kallen Stadtfeld in Code Geass, Nadja Applefield in Ashita no Nadja, Leonmitchelli Galette Des Rois in Dog Days, Yumi Hoshino in KimiKiss: Pure Rouge, Takuto Hasegawa in Magician's Academy, Hibiki Hojo in Suite PreCure, Nanaka Yatsushiro in Myself ; Yourself, Himari Noihara in Omamori Himari, Sailor Jupiter in Sailor Moon Crystal, Mikumo Guynemer in Macross Delta, Nodoka Haramura in Saki, Tenma Tsukamoto in School Rumble, Holo in Spice and Wolf, and Yang Xiao Long in both RWBY and RWBY: Ice Queendom. In video games, she voiced notable characters like Agnès Oblige in Bravely Default, Mai Shiranui since KOF Sky Stage, Mist in Rune Factory, Rimurisu in Tears to Tiara, Rinka in the Atelier series, Miyabi in Zenless Zone Zero, Rosa Ushiromiya in Umineko: Golden Fantasia, Tsuruhime in Sengoku Basara, Yukiko Amagi in Persona 4, Beidou in Genshin Impact, and Zima and Projekt Red in Arknights. She also is known for her dubbing role for Thailand films which starred Yanin Vismitananda. In addition, she also voiced in the live action anime film Shimajiro and the Rainbow Oasis.