Charles Town is a city in and the county seat of Jefferson County, West Virginia, United States. The population was 6,534 at the 2020 census. The city is named for its founder Charles Washington, youngest brother of President George Washington. It is part of the northwestern fringes of the Washington metropolitan area.

Jefferson County is located in the Shenandoah Valley in the Eastern Panhandle of West Virginia. It is the easternmost county of the U.S. state of West Virginia. As of the 2020 census, the population was 57,701. Its county seat is Charles Town. The county was founded in 1801, and today is part of the Washington metropolitan area.