Glycolysis is the metabolic pathway that converts glucose C6H12O6, into pyruvate, CH3COCOO− + H+. The free energy released in this process is used to form the high-energy molecules ATP and NADH. Glycolysis is a sequence of ten enzyme-catalyzed reactions. Most monosaccharides, such as fructose and galactose, can be converted to one of these intermediates; the intermediates may be directly useful. For example, the intermediate dihydroxyacetone phosphate is a source of the glycerol that combines with fatty acids to form fat. Glycolysis is an oxygen-independent metabolic pathway; the wide occurrence of glycolysis indicates. Indeed, the reactions that constitute glycolysis and its parallel pathway, the pentose phosphate pathway, occur metal-catalyzed under the oxygen-free conditions of the Archean oceans in the absence of enzymes. In most organisms, glycolysis occurs in the cytosol; the most common type of glycolysis is the Embden–Meyerhof–Parnas, discovered by Gustav Embden, Otto Meyerhof, Jakub Karol Parnas.
Glycolysis refers to other pathways, such as the Entner–Doudoroff pathway and various heterofermentative and homofermentative pathways. However, the discussion here will be limited to the Embden–Meyerhof–Parnas pathway; the glycolysis pathway can be separated into two phases: The Preparatory/Investment Phase – wherein ATP is consumed. The Pay Off Phase – wherein ATP is produced; the overall reaction of glycolysis is: The use of symbols in this equation makes it appear unbalanced with respect to oxygen atoms, hydrogen atoms, charges. Atom balance is maintained by the two phosphate groups: Each exists in the form of a hydrogen phosphate anion, dissociating to contribute 2 H+ overall Each liberates an oxygen atom when it binds to an ADP molecule, contributing 2 O overallCharges are balanced by the difference between ADP and ATP. In the cellular environment, all three hydroxyl groups of ADP dissociate into −O− and H+, giving ADP3−, this ion tends to exist in an ionic bond with Mg2+, giving ADPMg−.
ATP behaves identically except that it has four hydroxyl groups, giving ATPMg2−. When these differences along with the true charges on the two phosphate groups are considered together, the net charges of −4 on each side are balanced. For simple fermentations, the metabolism of one molecule of glucose to two molecules of pyruvate has a net yield of two molecules of ATP. Most cells will carry out further reactions to'repay' the used NAD+ and produce a final product of ethanol or lactic acid. Many bacteria use inorganic compounds as hydrogen acceptors to regenerate the NAD+. Cells performing aerobic respiration synthesize much more ATP, but not as part of glycolysis; these further aerobic reactions use pyruvate and NADH + H+ from glycolysis. Eukaryotic aerobic respiration produces 34 additional molecules of ATP for each glucose molecule, however most of these are produced by a vastly different mechanism to the substrate-level phosphorylation in glycolysis; the lower-energy production, per glucose, of anaerobic respiration relative to aerobic respiration, results in greater flux through the pathway under hypoxic conditions, unless alternative sources of anaerobically oxidizable substrates, such as fatty acids, are found.
The pathway of glycolysis as it is known today took 100 years to discover. The combined results of many smaller experiments were required in order to understand the pathway as a whole; the first steps in understanding glycolysis began in the nineteenth century with the wine industry. For economic reasons, the French wine industry sought to investigate why wine sometime turned distasteful, instead of fermenting into alcohol. French scientist Louis Pasteur researched this issue during the 1850s, the results of his experiments began the long road to elucidating the pathway of glycolysis, his experiments showed. While Pasteur's experiments were groundbreaking, insight into the component steps of glycolysis were provided by the non-cellular fermentation experiments of Eduard Buchner during the 1890s. Buchner demonstrated that the conversion of glucose to ethanol was possible using a non-living extract of yeast; this experiment not only revolutionized biochemistry, but allowed scientists to analyze this pathway in a more controlled lab setting.
In a series of experiments, scientists Arthur Harden and William Young discovered more pieces of glycolysis. They discovered the regulatory effects of ATP on glucose consumption during alcohol fermentation, they shed light on the role of one compound as a glycolysis intermediate: fructose 1,6-bisphosphate. The elucidation of fructose 1,6-bisphosphate was accomplished by measuring CO2 levels when yeast juice was incubated with glucose. CO2 production increased then slowed down. Harden and Young noted that this process would restart if an inorganic phosphate was added to the mixture. Harden and Young deduced that this process produced organic phosphate esters, further experiments allowed them to extract fructose diphosphate. Arthur Harden and William Young along with Nick Sheppard determined, in a second experiment, that a heat-sensitive high-molecular-weight subcellular fraction and a heat-insensitive low-molecular-weight cytoplasm fraction are required together for fermenta
El Comercio (Peru)
El Comercio is a Peru vian newspaper based in Lima. Founded in 1839, it is the oldest newspaper in Peru and one of the oldest Spanish-language papers in the world, it has a daily circulation of more than 120,000. It is one of the most influential media in Peru; the government of Juan Velasco Alvarado expropriated the newspaper in the mid-1970s. The company was returned to their original owners by President Fernando Belaúnde Terry on July 28, 1980, the same day he assumed office, it was his first official act upon assuming his presidency. The newspaper is owned by shareholders of the Miró Quesada family, whose ownership of the company dates to 1875. Despite this, management is under control of an individual, not a member of the family; the company has ownership over its subsidiaries, the newspapers Peru 21 and Trome, the magazine Somos. The corporation, Empresa Editora El Comercio S. A. is the product of the merging of many companies in 1996. The company manages the editing and distribution of the newspaper, El Comercio, as well as the publication and distribution of Trome, Peru 21, Gestion.
In addition, they manage the advertising aspects of the mentioned publications. Additionally, they are devoted to the editing and distribution of many other books, pamphlets, all sorts of graphic publications, multimedia products, videography. Informational content is distributed by their subsidiary Orbis Ventures S. A. C. A company in charge of the administration of the company's website; the legal address of the company, where their administrative offices are, is 300 Jr. Miró Quesada, Cercado of Lima, Peru, their publishing factories and Amauta, are in the districts of Pueblo Libre and the Cercado of Lima. Financially, the company operates independently, as the effects of consolidation have not in large part affected the operation of their subsidiaries, Orbis Ventures S. A. C. Zetta Comunicadores del Perú S. A. E. M. A. EC Jobs S. A. C. Punto y Coma Editores S. A. C. Suscripciones Integrales S. A. C. Amauta Impressiones Comerciales, Producciones Cantabria S. A. C. Inmobiliaria El Sol S. A. and Grupo TV S. A. C. In 1994, Ricardo Uceda resigned as editor-in-chief of Sí to form a special investigative team at El Comercio.
As with Uceda's Sí reporting, the Comercio team focused on cases of governmental corruption. One their most notable successes came in 1998, when they exposed the misuse of state funds intended for the survivors of floods and mudslides induced by the 1997-98 El Niño event. List of newspapers in Peru Media of Peru
A legislature is a deliberative assembly with the authority to make laws for a political entity such as a country or city. Legislatures form important parts of most governments. Laws enacted by legislatures are known as primary legislation. Legislatures observe and steer governing actions and have exclusive authority to amend the budget or budgets involved in the process; the members of a legislature are called legislators. In a democracy, legislators are most popularly elected, although indirect election and appointment by the executive are used for bicameral legislatures featuring an upper chamber. Names for national legislatures include "parliament", "congress", "diet", "assembly", depending on country; each chamber of the legislature consists of a number of legislators who use some form of parliamentary procedure to debate political issues and vote on proposed legislation. There must be a certain number of legislators present to carry out these activities; some of the responsibilities of a legislature, such as giving first consideration to newly proposed legislation, are delegated to committees made up of a few of the members of the chamber.
The members of a legislature represent different political parties. Legislatures vary in the amount of political power they wield, compared to other political players such as judiciaries and executives. In 2009, political scientists M. Steven Fish and Matthew Kroenig constructed a Parliamentary Powers Index in an attempt to quantify the different degrees of power among national legislatures; the German Bundestag, the Italian Parliament, the Mongolian State Great Khural tied for most powerful, while Myanmar's House of Representatives and Somalia's Transitional Federal Assembly tied for least powerful. Some political systems follow the principle of legislative supremacy, which holds that the legislature is the supreme branch of government and cannot be bound by other institutions, such as the judicial branch or a written constitution; such a system renders the legislature more powerful. In parliamentary and semi-presidential systems of government, the executive is responsible to the legislature, which may remove it with a vote of no confidence.
On the other hand, according to the separation of powers doctrine, the legislature in a presidential system is considered an independent and coequal branch of government along with both the judiciary and the executive. Legislatures will sometimes delegate their legislative power to administrative or executive agencies. Legislatures are made up of individual members, known as legislators. A legislature contains a fixed number of legislators. For example, a legislature that has 100 "seats" has 100 members. By extension, an electoral district that elects a single legislator can be described as a "seat", as, example, in the phrases "safe seat" and "marginal seat". A legislature may debate and vote upon bills as a single unit, or it may be composed of multiple separate assemblies, called by various names including legislative chambers, debate chambers, houses, which debate and vote separately and have distinct powers. A legislature which operates as a single unit is unicameral, one divided into two chambers is bicameral, one divided into three chambers is tricameral.
In bicameral legislatures, one chamber is considered the upper house, while the other is considered the lower house. The two types are not rigidly different, but members of upper houses tend to be indirectly elected or appointed rather than directly elected, tend to be allocated by administrative divisions rather than by population, tend to have longer terms than members of the lower house. In some systems parliamentary systems, the upper house has less power and tends to have a more advisory role, but in others presidential systems, the upper house has equal or greater power. In federations, the upper house represents the federation's component states; this is a case with the supranational legislature of the European Union. The upper house may either contain the delegates of state governments – as in the European Union and in Germany and, before 1913, in the United States – or be elected according to a formula that grants equal representation to states with smaller populations, as is the case in Australia and the United States since 1913.
Tricameral legislatures are rare. Tetracameral legislatures no longer exist, but they were used in Scandinavia. Legislatures vary in their size. Among national legislatures, China's National People's Congress is the largest with 2 980 members, while Vatican City's Pontifical Commission is the smallest with 7. Neither legislature is democratically elected: the National People's Congress is indirectly elected. Legislature size is a trade off between representation. Comparative analysis of national legislatures has found that size of a country's lower house tends to be proportional to the cube root of its population.
Deoxyribonucleic acid is a molecule composed of two chains that coil around each other to form a double helix carrying the genetic instructions used in the growth, development and reproduction of all known organisms and many viruses. DNA and ribonucleic acid are nucleic acids; the two DNA strands are known as polynucleotides as they are composed of simpler monomeric units called nucleotides. Each nucleotide is composed of one of four nitrogen-containing nucleobases, a sugar called deoxyribose, a phosphate group; the nucleotides are joined to one another in a chain by covalent bonds between the sugar of one nucleotide and the phosphate of the next, resulting in an alternating sugar-phosphate backbone. The nitrogenous bases of the two separate polynucleotide strands are bound together, according to base pairing rules, with hydrogen bonds to make double-stranded DNA; the complementary nitrogenous bases are divided into two groups and purines. In DNA, the pyrimidines are cytosine. Both strands of double-stranded DNA store the same biological information.
This information is replicated as and when the two strands separate. A large part of DNA is non-coding, meaning that these sections do not serve as patterns for protein sequences; the two strands of DNA are thus antiparallel. Attached to each sugar is one of four types of nucleobases, it is the sequence of these four nucleobases along the backbone. RNA strands are created using DNA strands as a template in a process called transcription. Under the genetic code, these RNA strands specify the sequence of amino acids within proteins in a process called translation. Within eukaryotic cells, DNA is organized into long structures called chromosomes. Before typical cell division, these chromosomes are duplicated in the process of DNA replication, providing a complete set of chromosomes for each daughter cell. Eukaryotic organisms store most of their DNA inside the cell nucleus as nuclear DNA, some in the mitochondria as mitochondrial DNA, or in chloroplasts as chloroplast DNA. In contrast, prokaryotes store their DNA only in circular chromosomes.
Within eukaryotic chromosomes, chromatin proteins, such as histones and organize DNA. These compacting structures guide the interactions between DNA and other proteins, helping control which parts of the DNA are transcribed. DNA was first isolated by Friedrich Miescher in 1869, its molecular structure was first identified by Francis Crick and James Watson at the Cavendish Laboratory within the University of Cambridge in 1953, whose model-building efforts were guided by X-ray diffraction data acquired by Raymond Gosling, a post-graduate student of Rosalind Franklin. DNA is used by researchers as a molecular tool to explore physical laws and theories, such as the ergodic theorem and the theory of elasticity; the unique material properties of DNA have made it an attractive molecule for material scientists and engineers interested in micro- and nano-fabrication. Among notable advances in this field are DNA origami and DNA-based hybrid materials. DNA is a long polymer made from repeating units called nucleotides.
The structure of DNA is dynamic along its length, being capable of coiling into tight loops and other shapes. In all species it is composed of two helical chains, bound to each other by hydrogen bonds. Both chains are coiled around the same axis, have the same pitch of 34 angstroms; the pair of chains has a radius of 10 angstroms. According to another study, when measured in a different solution, the DNA chain measured 22 to 26 angstroms wide, one nucleotide unit measured 3.3 Å long. Although each individual nucleotide is small, a DNA polymer can be large and contain hundreds of millions, such as in chromosome 1. Chromosome 1 is the largest human chromosome with 220 million base pairs, would be 85 mm long if straightened. DNA does not exist as a single strand, but instead as a pair of strands that are held together; these two long strands coil in the shape of a double helix. The nucleotide contains both a segment of the backbone of a nucleobase. A nucleobase linked to a sugar is called a nucleoside, a base linked to a sugar and to one or more phosphate groups is called a nucleotide.
A biopolymer comprising multiple linked nucleotides is called a polynucleotide. The backbone of the DNA strand is made from alternating sugar residues; the sugar in DNA is 2-deoxyribose, a pentose sugar. The sugars are joined together by phosphate groups that form phosphodiester bonds between the third and fifth carbon atoms of adjacent sugar rings; these are known as the 3′-end, 5′-end carbons, the prime symbol being used to distinguish these carbon atoms from those of the base to which the deoxyribose forms a glycosidic bond. When imagining DNA, each phosphoryl is considered to "belong" to the nucleotide whose 5′ carbon forms a bond therewith. Any DNA strand therefore has one end at which there is a phosphoryl attached to the 5′ carbon of a ribose and another end a
University of North Carolina at Chapel Hill
The University of North Carolina at Chapel Hill known as UNC-Chapel Hill, Chapel Hill, North Carolina, or Carolina is a public research university in Chapel Hill, North Carolina. It is the flagship of the 17 campuses of the University of North Carolina system. After being chartered in 1789, the university first began enrolling students in 1795, which allows it to be one of three schools to claim the title of the oldest public university in the United States. Among the claimants, the University of North Carolina at Chapel Hill is the only one to have held classes and graduated students as a public university in the eighteenth century; the first public institution of higher education in North Carolina, the school opened its doors to students on February 12, 1795. The university offers degrees in over 70 courses of study through fourteen colleges and the College of Arts and Sciences. All undergraduates receive a liberal arts education and have the option to pursue a major within the professional schools of the university or within the College of Arts and Sciences from the time they obtain junior status.
Under the leadership of President Kemp Plummer Battle, in 1877 North Carolina became coeducational and began the process of desegregation in 1951 when African-American graduate students were admitted under Chancellor Robert Burton House. In 1952, North Carolina opened its own hospital, UNC Health Care, for research and treatment, has since specialized in cancer care; the school's students and sports teams are known as "Tar Heels". UNC's faculty and alumni include 9 Nobel Prize laureates, 23 Pulitzer Prize winners, 49 Rhodes Scholars. Additional notable alumni include a U. S. President, a U. S. Vice President, 38 Governors of U. S. States, 98 members of the United States Congress, 9 Cabinet members, 39 Henry Luce Scholars, 9 World Cup winners and 3 astronauts as well as founders and CEOs of Fortune 500 companies; the campus covers 729 acres of Chapel Hill's downtown area, encompassing the Morehead Planetarium and the many stores and shops located on Franklin Street. Students can participate in over 550 recognized student organizations.
The student-run newspaper The Daily Tar Heel has won national awards for collegiate media, while the student radio station WXYC provided the world's first internet radio broadcast. In 2018, UNC was ranked amongst the top 30 universities in the United States according to the Academic Ranking of World Universities, Washington Monthly, U. S. News & World Report. Internationally, UNC is ranked 33rd and 34th in the world by Academic Ranking of World Universities and U. S. News and World Report, respectively. UNC is regarded as a Public Ivy, an institution which provides an Ivy League collegiate experience at a public school price. North Carolina is one of the charter members of the Atlantic Coast Conference, founded on June 14, 1953. Competing athletically as the Tar Heels, North Carolina has achieved great success in sports, most notably in men's basketball, women's soccer, women's field hockey. Chartered by the North Carolina General Assembly on December 11, 1789, the university's cornerstone was laid on October 12, 1793, near the ruins of a chapel, chosen because of its central location within the state.
The first public university chartered under the US Constitution, The University of North Carolina at Chapel Hill is one of three universities that claims to be the oldest public university in the United States and the only such institution to confer degrees in the eighteenth century as a public institution. During the Civil War, North Carolina Governor David Lowry Swain persuaded Confederate President Jefferson Davis to exempt some students from the draft, so the university was one of the few in the Confederacy that managed to stay open. However, Chapel Hill suffered the loss of more of its population during the war than any village in the South, when student numbers did not recover, the university was forced to close during Reconstruction from December 1, 1870 until September 6, 1875. Despite initial skepticism from university President Frank Porter Graham, on March 27, 1931, legislation was passed to group the University of North Carolina with the State College of Agriculture and Engineering and Woman's College of the University of North Carolina to form the Consolidated University of North Carolina.
In 1963, the consolidated university was made coeducational, although most women still attended Woman's College for their first two years, transferring to Chapel Hill as juniors, since freshmen were required to live on campus and there was only one women's residence hall. As a result, Woman's College was renamed the "University of North Carolina at Greensboro", the University of North Carolina became the "University of North Carolina at Chapel Hill." In 1955, UNC Chapel Hill desegregated its undergraduate divisions. During World War II, UNC Chapel Hill was one of 131 colleges and universities nationally that took part in the V-12 Navy College Training Program which offered students a path to a Navy commission. During the 1960s, the campus was the location of significant political protest. Prior to the passage of the Civil Rights Act of 1964, protests about local racial segregation which began in Franklin Street restaurants led to mass demonstrations and disturbance; the climate of civil unrest prompted the 1963 Speaker Ban Law prohibiting speeches by communists on state campuses in North Carolina.
The law was criticized by university Chancellor William Brantley Aycock and university President William Friday, but was not reviewed by the North Carolina General Assembly until 1965. Small amendments to allow "infrequent" visits failed to placate the student body when the university's board of trustees overruled new Chancellor Paul Frederick Sh
A hexokinase is an enzyme that phosphorylates hexoses, forming hexose phosphate. In most organisms, glucose is the most important substrate of hexokinases, glucose-6-phosphate is the most important product. Hexokinase possesses the ability to transfer an inorganic phosphate group from ATP to a substrate. Hexokinases should not be confused with glucokinase, a specific isoform of hexokinase. All hexokinases are capable of phosphorylating several hexoses but glucokinase acts with a 50-fold lower substrate affinity and its main hexose substrate is glucose. Genes that encode hexokinase have been discovered in every domain of life, exist among a variety of species that range from bacteria and plants to humans and other vertebrates, they are categorized as actin fold proteins, sharing a common ATP binding site core, surrounded by more variable sequences which determine substrate affinities and other properties. Several hexokinase isoforms or isozymes that provide different functions can occur in a single species.
The intracellular reactions mediated by hexokinases can be typified as: Hexose-CH2OH + MgATP2− → Hexose-CH2O-PO2−3 + MgADP− + H+where hexose-CH2OH represents any of several hexoses that contain an accessible -CH2OH moiety. Phosphorylation of a hexose such as glucose limits it to a number of intracellular metabolic processes, such as glycolysis or glycogen synthesis; this is because phosphorylated hexoses are charged, thus more difficult to transport out of a cell. In patients with essential fructosuria, metabolism of fructose by hexokinase to fructose-6-phosphate is the primary method of metabolizing dietary fructose. Most bacterial hexokinases are 50 kD in size. Multicellular organisms including plants and animals have more than one hexokinase isoform. Most consist of two halves, which share much sequence homology; this suggests an evolutionary origin by duplication and fusion of a 50kD ancestral hexokinase similar to those of bacteria. There are four important mammalian hexokinase isozymes that vary in subcellular locations and kinetics with respect to different substrates and conditions, physiological function.
They are designated hexokinases I, II, III, IV or hexokinases A, B, C, D. Hexokinases I, II, III are referred to as "low-Km" isozymes because of a high affinity for glucose. Hexokinases I and II follow Michaelis-Menten kinetics at physiologic concentrations of substrates. All three are inhibited by their product, glucose-6-phosphate. Molecular weights are around 100 kD; each consists of two similar 50kD halves, but only in hexokinase II do both halves have functional active sites. Hexokinase I/A is found in all mammalian tissues, is considered a "housekeeping enzyme," unaffected by most physiological and metabolic changes. Hexokinase II/B constitutes the principal regulated isoform in many cell types and is increased in many cancers, it is the hexokinase found in heart. Hexokinase II is located at the mitochondria outer membrane so it can have direct access to ATP. Hexokinase III/C is substrate-inhibited by glucose at physiologic concentrations. Little is known about the regulatory characteristics of this isoform.
Mammalian hexokinase IV referred to as glucokinase, differs from other hexokinases in kinetics and functions. The location of the phosphorylation on a subcellular level occurs when glucokinase translocates between the cytoplasm and nucleus of liver cells. Glucokinase can only phosphorylate glucose. Hexokinase IV is monomeric, about 50kD, displays positive cooperativity with glucose, is not allosterically inhibited by its product, glucose-6-phosphate. Hexokinase IV is present in the liver, hypothalamus, small intestine, certain other neuroendocrine cells, plays an important regulatory role in carbohydrate metabolism. In the beta cells of the pancreatic islets, it serves as a glucose sensor to control insulin release, controls glucagon release in the alpha cells. In hepatocytes of the liver, glucokinase responds to changes of ambient glucose levels by increasing or reducing glycogen synthesis. Glucose is unique in that it can be used to produce ATP by all cells in both the presence and absence of molecular oxygen.
The first step in glycolysis is the phosphorylation of glucose by hexokinase. Compound C00031 at KEGG Pathway Database. Enzyme 22.214.171.124 at KEGG Pathway Database. Compound C00668 at KEGG Pathway Database. Reaction R01786 at KEGG Pathway Database. By catalyzing the phosphorylation of glucose to yield glucose 6-phosphate, hexokinases maintain the downhill concentration gradient that favors the facilitated transport of glucose into cells; this reaction initiates all physiologically relevant pathways of glucose utilization, including glycolysis and the pentose phosphate pathway. The addition of a charged phosphate group at the 6-position of hexoses ensures'trapping' of glucose and 2-deoxyhexose glucose analogs within cells, as charged hexose phosphates cannot cross the cell membrane. Hexokinases I and II can associate physically to the outer surface of the external membrane of mitochondria through specific binding to a porin, or voltage dependent anion channel; this association confers hexokinase direct access to ATP generated by mitochondria, one of the two substrates of hexokinase.
Mitochondrial hexokinase is elevated in growing malignant tumor cells, with levels up to 200 times higher than normal tissues
Lima is the capital and the largest city of Peru. It is located in the valleys of the Chillón, Rímac and Lurín rivers, in the central coastal part of the country, overlooking the Pacific Ocean. Together with the seaport of Callao, it forms a contiguous urban area known as the Lima Metropolitan Area. With a population of more than 9 million, Lima is the most populous metropolitan area of Peru and the third-largest city in the Americas, behind São Paulo and Mexico City. Lima was founded by Spanish conquistador Francisco Pizarro on 18 January 1535, as Ciudad de los Reyes in the agricultural region known by the Indians as Limaq, name that acquired over time, it became most important city in the Viceroyalty of Peru. Following the Peruvian War of Independence, it became the capital of the Republic of Peru. Around one-third of the national population lives in the metropolitan area. Lima is home to one of the oldest institutions of higher learning in the New World; the National University of San Marcos, founded on 12 May 1551, during the Spanish colonial regime, is the oldest continuously functioning university in the Americas.
Nowadays the city is considered as the political, cultural and commercial center of the country. Internationally, it is one of the thirty most populated urban agglomerations in the world. Due to its geostrategic importance, it has been defined as a "beta" city. Jurisdictionally, the metropolis extends within the province of Lima and in a smaller portion, to the west, within the constitutional province of Callao, where the seaport and the Jorge Chávez airport are located. Both provinces have regional autonomy since 2002. In October 2013, Lima was chosen to host the 2019 Pan American Games, it hosted the United Nations Climate Change Conference in December 2014 and the Miss Universe 1982 contest. According to early Spanish articles the Lima area was once called Itchyma, after its original inhabitants; however before the Inca occupation of the area in the 15th century, a famous oracle in the Rímac valley had come to be known by visitors as Limaq. This oracle was destroyed by the Spanish and replaced with a church, but the name persisted: the chronicles show "Límac" replacing "Ychma" as the common name for the area.
Modern scholars speculate that the word "Lima" originated as the Spanish pronunciation of the native name Limaq. Linguistic evidence seems to support this theory as spoken Spanish rejects stop consonants in word-final position. Non-Peruvian Spanish speakers may mistakenly define the city name as the direct Spanish translation of "lime", the citrus fruit; the city was founded in 1535 under the name City of the Kings because its foundation was decided on 6 January, date of the feast of the Epiphany. This name fell into disuse and Lima became the city's name of choice; the river that feeds Lima is called Rímac and many people erroneously assume that this is because its original Inca name is "Talking River". However, the original inhabitants of the valley were not Incas; this name is an innovation arising from an effort by the Cuzco nobility in colonial times to standardize the toponym so that it would conform to the phonology of Cuzco Quechua. As the original inhabitants died out and the local Quechua became extinct, the Cuzco pronunciation prevailed.
Nowadays, Spanish-speaking locals do not see the connection between the name of their city and the name of the river that runs through it. They assume that the valley is named after the river; the Flag of Lima has been known as the "Banner of Peru's Kings' City". It is embroidered in the center is its coat of arms. Lima's anthem was heard for the first time on 18 January 2008, in a formal meeting with important politicians, including Peruvian President Alan García, other authorities; the anthem was created by Euding Maeshiro and record producer Ricardo Núñez. In the pre-Columbian era, what is now Lima was inhabited by indigenous groups under the Ychsma policy, incorporated into the Inca Empire in the 15th century. In 1532 a group of Spanish conquistadors, led by Francisco Pizarro, defeated the Inca ruler Atahualpa and took over his empire; as the Spanish Crown had named Pizarro governor of the lands he conquered, he chose the Rímac Valley to found his capital on 18 January 1535, as Ciudad de los Reyes.
In August 1536, rebel Inca troops led by Manco Inca Yupanqui besieged the city but were defeated by the Spaniards and their native allies. Lima gained prestige after being designated capital of the Viceroyalty of Peru and site of a Real Audiencia in 1543. During the next century it flourished as the centre of an extensive trade network that integrated the Viceroyalty with the rest of the Americas and the Far East. However, the city was not free from dangers; the 1687 Peru earthquake destroyed most of the city buildings. In 1746, another p