Exo-(1-4)-α-D-glucan lyase

From Wikipedia, the free encyclopedia
Jump to navigation Jump to search
Exo-(1->4)-alpha-D-glucan lyase
EC number
CAS number 148710-18-3
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum

Exo-(1->4)-alpha-D-glucan lyase (EC, alpha-(1->4)-glucan 1,5-anhydro-D-fructose eliminase, alpha-1,4-glucan exo-lyase, alpha-1,4-glucan lyase, GLase) is an enzyme with systematic name (1->4)-alpha-D-glucan exo-4-lyase (1,5-anhydro-D-fructose-forming).[1][2][3][4][5][6][7] This enzyme catalyses the following chemical reaction

linear alpha-glucan (n-1) 1,5-anhydro-D-fructose + D-glucose

The enzyme catalyses the sequential degradation of (1->4)-alpha-D-glucans from the non-reducing end.


  1. ^ Yu, S.; Kenne, L.; Pedersén, M. (1993). "α-1,4-Glucan lyase, a new class of starch/glycogen degrading enzyme. I. Efficient purification and characterization from red seaweeds". Biochim. Biophys. Acta. 1156 (3): 313–320. doi:10.1016/0304-4165(93)90049-e. PMID 8461323. 
  2. ^ Yu, S.; Pedersén, M. (1993). "α-1,4-Glucan lyase, a new class of starch/glycogen degrading enzyme. II. Subcellular localization and partial amino-acid sequence". Planta. 191 (1): 137–142. doi:10.1007/bf00240905. PMID 7763826. 
  3. ^ Yu, S.; Ahmad, T.; Kenne, L.; Pedersén, M. (1995). "α-1,4-Glucan lyase, a new class of starch/glycogen degrading enzyme. III. Substrate specificity, mode of action, and cleavage mechanism". Biochim. Biophys. Acta. 1244 (1): 1–9. doi:10.1016/0304-4165(94)00202-9. PMID 7766642. 
  4. ^ Yu, S.; Christensen, T.M.; Kragh, K.M.; Bojsen, K.; Marcussen, J. (1997). "Efficient purification, characterization and partial amino acid sequencing of two α-1,4-glucan lyases from fungi". Biochim. Biophys. Acta. 1339 (2): 311–320. doi:10.1016/s0167-4838(97)00014-9. PMID 9187252. 
  5. ^ Yu, S.; Bojsen, K.; Svensson, B.; Marcussen, J. (1999). "α-1,4-glucan lyases producing 1,5-anhydro-D-fructose from starch and glycogen have sequence similarity to α-glucosidases". Biochim. Biophys. Acta. 1433 (1–2): 1–15. doi:10.1016/s0167-4838(99)00152-1. PMID 10446355. 
  6. ^ Lee, S.S.; Yu, S.; Withers, S.G. (2002). "α-1,4-Glucan lyase performs a trans-elimination via a nucleophilic displacement followed by a syn-elimination". J. Am. Chem. Soc. 124 (18): 4948–4949. doi:10.1021/ja0255610. PMID 11982345. 
  7. ^ Lee, S.S.; Yu, S.; Withers, S.G. (2003). "Detailed dissection of a new mechanism for glycoside cleavage: α-1,4-glucan lyase". Biochemistry. 42 (44): 13081–13090. doi:10.1021/bi035189g. PMID 14596624. 

External links[edit]