Folin's reagent

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Folin's reagent
Skeletal formula of Folin's reagent
Space-filling model of the Folin's reagent molecule
IUPAC name
Sodium 3,4-dioxo-3,4-dihydronaphthalene-1-sulfonate
3D model (JSmol)
ECHA InfoCard 100.007.555
EC Number 208-308-9
Molar mass 260.20 g/mol
Safety data sheet Oxford MSDS
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Infobox references

Folin's reagent or sodium 1,2-naphthoquinone-4-sulfonate is a chemical reagent used as a derivatizing agent to measure levels of amines and amino acids.[1] The reagent reacts with them in alkaline solution to produce a fluorescent material that can be easily detected.[2]

Folin reaction.png

This should not be confused with Folin-Ciocalteu reagent, that is used to detect phenolic compounds.

The Folin reagent can be used with an acidic secondary reagent to distinguish MDMA and related compounds from PMMA and related compounds.[3][not in citation given]

Substance Reaction
MDMA Pink[3]
MDE No Reaction[3]
MDA Yellow[3]
BZP Orange to red[3]
TFMPP Pinkish red[3]
Methylone No reaction[3]
Methamphetamine Pink[3]
Amphetamine Light orange[3]
PMA Light Yellow[3]
PMMA Light orange[3]
Ketamine No reaction[3]
Mescaline No reaction[3]
Cocaine No reaction[3]
Aspirin No reaction[3]
Sugar No reaction[3]

See also[edit]


  1. ^ Saurina J, Hernández-Cassou S (1994). "Determination of amino acids by ion-pair liquid chromatography with post-column derivatization using 1,2-naphthoquinone-4-sulfonate". Journal of Chromatography A. 676 (2): 311–9. doi:10.1016/0021-9673(94)80431-1. PMID 7921184. 
  2. ^ Kobayashi Y, Kubo H, Kinoshita T (1987). "Fluorometric determination of guanidino compounds by new postcolumn derivatization system using reversed-phase ion-pair high-performance liquid chromatography". Anal. Biochem. 160 (2): 392–8. doi:10.1016/0003-2697(87)90066-2. PMID 3578768. 
  3. ^ a b c d e f g h i j k l m n o p "Folin Reagent Testing Kit". Dancesafe. June 2015. Retrieved 8 August 2015.