Germacrene-A synthase

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Germacrene-A synthase
Identifiers
EC number4.2.3.23
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

In enzymology, a germacrene-A synthase (EC 4.2.3.23) is an enzyme that catalyzes the chemical reaction

2-trans,6-trans-farnesyl diphosphate (+)-(R)-gemacrene A + diphosphate

Hence, this enzyme has one substrate, 2-trans,6-trans-farnesyl diphosphate, and two products, (+)-(R)-gemacrene A and diphosphate.

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase [(+)-(R)-germacrene-A-forming]. Other names in common use include germacrene A synthase, (+)-germacrene A synthase, (+)-(10R)-germacrene A synthase, GAS, 2-trans,6-trans-farnesyl-diphosphate diphosphate-lyase, and (germacrene-A-forming).

References[edit]

  • Wallaart TE; Kodde, J; Verstappen, FW; Altug, IG; De Kraker, JW; Wallaart, TE (2002). "Isolation and Characterization of Two Germacrene A Synthase cDNA Clones from Chicory". Plant Physiol. 129 (1): 134&ndash, 44. doi:10.1104/pp.001024. PMC 155878. PMID 12011345.
  • Beale MH; Phillips, AL; Gittings, S; Lewis, MJ; Hooper, AM; Pickett, JA; Beale, MH (2002). "(+)-(10R)-Germacrene A synthase from goldenrod, Solidago canadensis cDNA isolation, bacterial expression and functional analysis". Phytochemistry. 60 (7): 691&ndash, 702. doi:10.1016/S0031-9422(02)00165-6. PMID 12127586.
  • de Kraker JW, Franssen MC, de Groot A, Konig WA, Bouwmeester HJ (1998). "(+)-Germacrene A Biosynthesis : The Committed Step in the Biosynthesis of Bitter Sesquiterpene Lactones in Chicory". Plant Physiol. 117 (4): 1381&ndash, 92. doi:10.1104/pp.117.4.1381. PMC 34902. PMID 9701594.
  • Calvert MJ, Ashton PR, Allemann RK (2002). "Germacrene A is a product of the aristolochene synthase-mediated conversion of farnesylpyrophosphate to aristolochene". J. Am. Chem. Soc. 124 (39): 11636&ndash, 41. doi:10.1021/ja020762p. PMID 12296728.
  • Chang YJ, Jin J, Nam HY, Kim SU (2005). "Point mutation of (+)-germacrene A synthase from Ixeris dentata". Biotechnol. Lett. 27 (5): 285&ndash, 8. doi:10.1007/s10529-005-0681-9. PMID 15834787.