John Chambers was a U.S. Representative from Kentucky and the second Governor of the Iowa Territory. He was appointed by President William Henry Harrison.

The Territory of Iowa was an organized incorporated territory of the United States that existed from July 4, 1838, until December 28, 1846, when the southeastern portion of the territory was admitted to the Union as the state of Iowa. The remainder of the territory would have no organized territorial government until the Minnesota Territory was organized on March 3, 1849.