John Ruthven, 3rd Earl of Gowrie, was a Scottish nobleman who died in mysterious circumstances, referred to as the "Gowrie Conspiracy", in which he and/or his brother Alexander were attempting to kill or kidnap King James VI of Scotland for unknown purposes. The king's retinue killed both brothers during the attack, and the king survived.

James VI and I was King of Scotland as James VI from 24 July 1567 and King of England and Ireland as James I from the union of the Scottish and English crowns on 24 March 1603 until his death in 1625. Although he long tried to get both countries to adopt a closer political union, the kingdoms of Scotland and England remained sovereign states, with their own parliaments, judiciaries, and laws, ruled by James in personal union. He was the longest-reigning monarch of the Kingdom of Scotland.