The Leader of the Opposition in Queensland is the title of the leader of the largest minority political party or coalition of parties, known as the Opposition, in the Parliament of Queensland. Prior to 1898, opposition to the government of the day was less organised. Thus the Queensland Parliamentary Record does not designate Leaders of the Opposition before then. The Leader is responsible for managing the Opposition and has a role in administering the Legislative Assembly through the Committee of the Legislative Assembly.

The Parliament of Queensland is the legislature of Queensland, Australia. As provided under the Constitution of Queensland, the Parliament consists of the King and the Legislative Assembly. It has been the only unicameral state legislature in the country since the upper chamber, the Legislative Council, was abolished in 1922. The Legislative Assembly sits in Parliament House in the state capital, Brisbane.