Mandelate 4-monooxygenase

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mandelate 4-monooxygenase
EC number
CAS number 39459-82-0
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / QuickGO

In enzymology, a mandelate 4-monooxygenase (EC is an enzyme that catalyzes the chemical reaction

(S)-2-hydroxy-2-phenylacetate + tetrahydrobiopterin + O2 (S)-4-hydroxymandelate + dihydrobiopterin + H2O

The 3 substrates of this enzyme are (S)-2-hydroxy-2-phenylacetate, tetrahydrobiopterin, and O2, whereas its 3 products are (S)-4-hydroxymandelate, dihydrobiopterin, and H2O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with reduced pteridine as one donor, and incorporation of one ato of oxygen into the other donor. The systematic name of this enzyme class is (S)-2-hydroxy-2-phenylacetate,tetrahydrobiopterin:oxygen oxidoreductase (4-hydroxylating). Other names in common use include L-mandelate 4-hydroxylase, and mandelic acid 4-hydroxylase. It employs one cofactor, iron.


  • Bhat SG, Vaidyanathan CS (1976). "Purifications and properties of L-mandelate- 4-hydroxylase from Pseudomonas convexa". Arch. Biochem. Biophys. 176 (1): 314–23. doi:10.1016/0003-9861(76)90170-3. PMID 9909.