Opine dehydrogenase

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opine dehydrogenase
EC number
CAS number 108281-02-3
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / QuickGO

In enzymology, an opine dehydrogenase (EC is an enzyme that catalyzes the chemical reaction

(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate + NAD+ + H2O L-2-aminopentanoic acid + pyruvate + NADH + H+

The 3 substrates of this enzyme are [[(2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate]], NAD+, and H2O, whereas its 4 products are L-2-aminopentanoic acid, pyruvate, NADH, and H+.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate:NAD+ oxidoreductase (L-aminopentanoate-forming). Other names in common use include (2S)-2-{[1-(R)-carboxyethyl]amino}pentanoate dehydrogenase (NAD+,, and L-aminopentanoate-forming).


  • Asano Y, Yamaguchi K, Kondo K (1989). "A new NAD+-dependent opine dehydrogenase from Arthrobacter sp strain 1C". J. Bacteriol. 171 (8): 4466&ndash, 71. PMC 210226. PMID 2753861.
  • Dairi T, Asano Y (1995). "Cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from Arthrobacter sp. strain 1C". Appl. Environ. Microbiol. 61 (8): 3169&ndash, 71. PMC 167592. PMID 7487048.
  • Kato Y, Yamada H, Asano Y (1996). "Stereoselective synthesis of opine-type secondary amine carboxylic acids by a new enzyme opine dehydrogenase. Use of recombinant enzymes". J. Mol. Catal., B Enzym. 1 (3–6): 151&ndash, 160. doi:10.1016/1381-1177(95)00011-9.