The X-Files Mythology – Volume 3 collection is the third DVD release containing selected episodes from the fifth to the eighth seasons of the American science fiction television series The X-Files. The episodes collected in the release form the middle of the series' mythology, and are centered on alien colonization efforts, the fall of the Syndicate, and Fox Mulder's abduction.

The eighth season of the American science fiction television series The X-Files commenced airing in the United States on November 5, 2000, concluded on May 20, 2001, and consisted of twenty-one episodes. Season eight takes place after Fox Mulder's alien abduction in the seventh season. The story arc for the search of Mulder continues until the second half of the season, while a new arc about Dana Scully's pregnancy is formed. This arc would continue, and end, with the next season. The season explores various themes such as life, death, and belief.