Thiazole synthase

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Thiazole synthase
Thiazole synthase oktamer, Saccharomyces cerevisiae
EC number2.8.1.10
IntEnzIntEnz view
ExPASyNiceZyme view
MetaCycmetabolic pathway
PDB structuresRCSB PDB PDBe PDBsum

Thiazole synthase (EC, thiG (gene)) is an enzyme with systematic name 1-deoxy-D-xylulose 5-phosphate:thiol sulfurtransferase.[1][2][3][4][5][6] This enzyme catalyses the following chemical reaction

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] 2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O

H2S can provide the sulfur in vitro.


  1. ^ Park, J.H.; Dorrestein, P.C.; Zhai, H.; Kinsland, C.; McLafferty, F.W.; Begley, T.P. (2003). "Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1)". Biochemistry. 42: 12430–12438. doi:10.1021/bi034902z. PMID 14567704.
  2. ^ Dorrestein, P.C.; Zhai, H.; McLafferty, F.W.; Begley, T.P. (2004). "The biosynthesis of the thiazole phosphate moiety of thiamin: the sulfur transfer mediated by the sulfur carrier protein ThiS". Chem. Biol. 11: 1373–1381. doi:10.1016/j.chembiol.2004.08.009. PMID 15489164.
  3. ^ Dorrestein, P.C.; Zhai, H.; Taylor, S.V.; McLafferty, F.W.; Begley, T.P. (2004). "The biosynthesis of the thiazole phosphate moiety of thiamin (vitamin B1): the early steps catalyzed by thiazole synthase". J. Am. Chem. Soc. 126: 3091–3096. doi:10.1021/ja039616p. PMID 15012138.
  4. ^ Settembre, E.C.; Dorrestein, P.C.; Zhai, H.; Chatterjee, A.; McLafferty, F.W.; Begley, T.P.; Ealick, S.E. (2004). "Thiamin biosynthesis in Bacillus subtilis: structure of the thiazole synthase/sulfur carrier protein complex". Biochemistry. 43: 11647–11657. doi:10.1021/bi0488911. PMID 15362849.
  5. ^ Hazra, A.; Chatterjee, A.; Begley, T.P. (2009). "Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction". J. Am. Chem. Soc. 131: 3225–3229. doi:10.1021/ja806752h. PMC 2765510. PMID 19216519.
  6. ^ Hazra, A.B.; Han, Y.; Chatterjee, A.; Zhang, Y.; Lai, R.Y.; Ealick, S.E.; Begley, T.P. (2011). "A missing enzyme in thiamin thiazole biosynthesis: identification of TenI as a thiazole tautomerase". J. Am. Chem. Soc. 133: 9311–9319. doi:10.1021/ja1110514. PMC 3116082. PMID 21534620.

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