Tuskegee was an Overhill Cherokee town located along the lower Little Tennessee River in what is now Monroe County, Tennessee, United States. The town developed in the late 1750s alongside Fort Loudoun, and was inhabited until the late 1770s. It was forcibly evacuated and probably burned during the Cherokee–American wars.

Overhill Cherokee was the term for the Cherokee people located in their historic settlements in what is now the U.S. state of Tennessee in the Southeastern United States, on the western side of the Appalachian Mountains. This name was used by 18th-century European traders and explorers from British colonies along the Atlantic coast, as they had to cross the mountains to reach these settlements.