The 1964 Giro d'Italia was the 47th running of the Giro d'Italia, one of cycling's Grand Tour races. The Giro started in Bolzano, on 16 May, with a 173 km (107.5 mi) mass-start stage and concluded back in Milan, on 7 June, with a 146 km (90.7 mi) leg. A total of 130 riders from 13 teams entered the 22-stage race, which was won by Frenchman Jacques Anquetil of the Saint-Raphaël team. The second and third places were taken by Italian riders Italo Zilioli and Guido De Rosso, respectively.

The Giro d'Italia is an annual multiple-stage bicycle race primarily held in Italy, while also starting in, or passing through, other countries. The first race was organized in 1909 to increase sales of the newspaper La Gazzetta dello Sport, and the race is still run by a subsidiary of that paper's owner. The race has been held annually since its first edition in 1909, except during the two world wars. As the Giro gained prominence and popularity the race was lengthened, and the peloton expanded from primarily Italian participation to riders from all over the world. The Giro is a UCI World Tour event, which means that the teams that compete in the race are mostly UCI WorldTeams, with some additional teams invited as 'wild cards'.