The 2016 French Open was a tennis tournament played on outdoor clay courts. It was the 120th edition of the French Open and the second Grand Slam event of the year. It took place at the Stade Roland Garros from 22 May to 5 June and consisted of events for professional players in singles, doubles and mixed doubles play. Junior and wheelchair players also took part in singles and doubles events.

Garbiñe Muguruza defeated the defending champion Serena Williams in the final, 7–5, 6–4 to win the women's singles tennis title at the 2016 French Open. It was her first major singles title, and she became the second Spaniard to win the title. Muguruza lost only one set during the tournament, to Anna Karolína Schmiedlová in the first round. Williams was attempting to equal Steffi Graf's Open Era record of 22 major singles titles. She was also attempting to become only the second player, after Graf, to complete a quadruple career Grand Slam.