Bartram's Garden is a 50-acre public garden and National Historic Landmark in Southwest Philadelphia, Pennsylvania, situated on the banks of the Tidal Schuylkill River. Founded in 1728 by botanist John Bartram (1699–1777), it is the oldest botanical garden to survive in North America. The Garden is operated by the non-profit John Bartram Association in coordination with Philadelphia Parks and Recreation.

John Bartram was an American botanist, horticulturist, and explorer, based in Philadelphia, Pennsylvania, for most of his career. Swedish botanist and taxonomist Carl Linnaeus said he was the "greatest natural botanist in the world." Bartram corresponded with and shared North American plants and seeds with a variety of scientists in England and Europe.