Bison hunting was an activity fundamental to the economy and society of the Plains Indians peoples who inhabited the vast grasslands on the Interior Plains of North America, before the animal's near-extinction in the late 19th century following US expansion into the West. Bison hunting was an important spiritual practice and source of material for these groups, especially after the European introduction of the horse in the 16th through 19th centuries enabled new hunting techniques. The species' dramatic decline was the result of habitat loss due to the expansion of ranching and farming in western North America, industrial-scale hunting practiced by non-Indigenous hunters increased Indigenous hunting pressure due to non-Indigenous demand for bison hides and meat, and cases of a deliberate policy by settler governments to destroy the food source of the Indigenous peoples during times of conflict.

The American bison, also called the American buffalo or simply buffalo, is a species of bison native to North America. It is one of two extant species of bison, alongside the European bison. Its historical range circa 9000 BC is described as the great bison belt, a tract of rich grassland from Alaska to the Gulf of Mexico, east to the Atlantic Seaboard, as far north as New York, south to Georgia, and according to some sources, further south to northern Florida, with sightings in North Carolina near Buffalo Ford on the Catawba River as late as 1750.