Brother Blood is the name of two supervillains appearing in American comic books published by DC Comics. The first iteration, Sebastian Blood VIII, is a power-hungry priest and head of the Church of Blood, as well as the eighth person in the DC Universe to assume the mantle, after killing his father and taking the Brother Blood mantle from him. This tradition had gone on for generations, dating back to the 13th century, when the first Brother Blood was born after obtaining Jesus of Nazareth's prayer shawl and gaining superhuman abilities. As Brother Blood, Sebastian served as a recurring adversary of the Teen Titans, until being killed by his successor, Sebastian Blood IX.

The DC Universe (DCU) is the shared universe in which most stories in American comic book titles published by DC Comics take place. In context, the term "DC Universe" usually refers to the main DC continuity. It contains such well-known superheroes as Batman, Superman, Wonder Woman, the Flash, Green Lantern, Aquaman, Green Arrow, Shazam, Martian Manhunter, and Cyborg; as well as teams such as the Justice League, the Justice Society of America, the Suicide Squad, Doom Patrol, and the Teen Titans. It also contains well-known supervillains, including the Joker, Lex Luthor, the Cheetah, the Reverse-Flash, Sinestro, Black Manta, Deathstroke, Black Adam, Brainiac, and Darkseid.