Cowley County is a county located in the U.S. state of Kansas. Its county seat is Winfield, and its most populous city is Arkansas City. As of the 2020 census, the county population was 34,549. The county was named after Matthew Cowley, first lieutenant in Company I, 9th Kansas Cavalry, who died during the American Civil War.

Winfield is a city and county seat of Cowley County, Kansas, United States. It is situated along the Walnut River in South Central Kansas. As of the 2020 census, the population of the city was 11,777. It is home to Southwestern College.