The Czech Republic participated in the Eurovision Song Contest 2016 with the song "I Stand" written by Christian Schneider, Sara Biglert and Aidan O'Connor. The song was performed by Gabriela Gunčíková, who was internally selected by the Czech broadcaster Česká televize (ČT) to represent the nation at the 2016 contest in Stockholm, Sweden. Gunčíková and the song "I Stand" were announced as the Czech entry on 10 March 2016. The song was presented to the public on 11 March 2016.

The Eurovision Song Contest 2016 was the 61st edition of the Eurovision Song Contest. It took place in Stockholm, Sweden, following the country's victory at the 2015 contest with the song "Heroes" by Måns Zelmerlöw. Organised by the European Broadcasting Union (EBU) and host broadcaster Sveriges Television (SVT), the contest was held at the Globe Arena and consisted of two semi-finals on 10 and 12 May, and a final on 14 May 2016. The three live shows were presented by Petra Mede and the previous year's winner Måns Zelmerlöw.