Fort Caroline was an attempted French colonial settlement in Florida, located on the banks of the St. Johns River in present-day Duval County. It was established under the leadership of René Goulaine de Laudonnière on 22 June 1564, following King Charles IX's enlisting of Jean Ribault and his Huguenot settlers to stake a claim in French Florida ahead of Spain. The French colony came into conflict with the Spanish, who established St. Augustine in September 1565, and Fort Caroline was sacked by Spanish troops under Pedro Menéndez de Avilés on 20 September. The Spanish continued to occupy the site as San Mateo until 1569.

France began colonizing the Americas in the 16th century and continued into the following centuries as it established a colonial empire in the Western Hemisphere. France established colonies in much of eastern North America, on several Caribbean islands, and in South America. Most colonies were developed to export products such as fish, rice, sugar, and furs.