Gene targeting
Videos
Gene targeting is a biotechnological tool used to change the DNA sequence of an organism. It is based on the natural DNA-repair mechanism of homology directed repair, including homologous recombinatio
A chimeric mouse gene targeted for the agouti coat color gene, with its offspring
Wild-type Physcomitrella and knockout mosses: Deviating phenotypes induced in gene-disruption library transformants. Physcomitrella wild-type and transformed plants were grown on minimal Knop medium to induce differentiation and development of gametophores. For each plant, an overview (upper row, scale bar corresponds to 1 mm) and a close-up (bottom row, scale bar equals 0.5 mm) is shown. A, Haploid wild-type moss plant completely covered with leafy gametophores and close-up of wild-type leaf. B–E, Different mutants.
Physcomitrella patens
Videos
Physcomitrella patens is a synonym of Physcomitrium patens, the spreading earthmoss. It is a moss, a bryophyte used as a model organism for studies on plant evolution, development, and physiology.
Gene targeting
…make up the reproductive organs are bred. After this step the entire body of the mouse is based on the selected embryonic stem cell. To target genes in moss, the DNA is incubated together with freshly isolated protoplasts and with polyethylene glycol. As mosses are haploid organisms, moss filaments (protonema…
Physcomitrella
Cleistocarpous sporophyte of the moss P. patens
P. patens plants growing axenically in vitro on agar plates (Petri dish, 9 cm diameter)
Moss bioreactor with P. patens