Jamestown 2007 is the name of the organization which planned the events commemorating the 400th anniversary (quadricentennial) of the founding of Jamestown, Virginia in 1607, the first permanent English-speaking settlement in what is now the United States of America. America's 400th Anniversary was an 18-month-long commemoration including 10 Signature Events, hundreds of community programs and dozens of partner and programs and events. Activities took place throughout Virginia, in major cities along the East Coast, and in the United Kingdom.

The Jamestown settlement in the Colony of Virginia was the first permanent English settlement in the Americas. It was located on the northeast bank of the James River, about 2.5 mi (4 km) southwest of the center of modern Williamsburg. It was established by the Virginia Company of London as "James Fort" on May 4, 1607 O.S., and was considered permanent after a brief abandonment in 1610. It followed several failed attempts, including the Lost Colony of Roanoke, established in 1585 on Roanoke Island, later part of North Carolina. Jamestown served as the colonial capital from 1616 until 1699. Despite the dispatch of more settlers and supplies, more than 80 percent of the colonists died in 1609–1610, mostly from starvation and disease. In mid-1610, the survivors abandoned Jamestown, though they returned after meeting a resupply convoy in the James River.