Jan Evangelista Purkyně was a Czech anatomist and physiologist. In 1839, he coined the term "protoplasma" for the fluid substance of a cell. He was one of the best known scientists of his time. Such was his fame that when people from outside Europe wrote letters to him, all that they needed to put as the address was "Purkyně, Europe".

Charles University, also known as Charles University in Prague or historically as the University of Prague, is the oldest and largest university in the Czech Republic, the second university established in the Holy Roman Empire after Bologna. It is one of the oldest universities in the world in continuous operation, the first university north of the Alps and east of Paris. Today, the university consists of 17 faculties located in Prague, Hradec Králové, and Plzeň. Charles University is in the top three universities in Central and Eastern Europe. The institution has been ranked 200–300 in the world as one of the best universities.