The Lamborghini V12 refers to the flagship V12 engine used by Lamborghini. Lamborghini has had two generations of V12 engines through their history, both of which were developed in-house. The first-generation Lamborghini V12 was a sixty degree (60°) V12 petrol engine designed by Lamborghini, and was the first internal combustion engine ever produced by the firm.

A V12 engine is a twelve-cylinder piston engine where two banks of six cylinders are arranged in a V configuration around a common crankshaft. V12 engines are more common than V10 engines. However, they are less common than V8 engines.