Muscatine is a city in Muscatine County, Iowa, United States. The population was 23,797 at the time of the 2020 census, an increase from 22,697 in 2000. The county seat of Muscatine County, it is located along the Mississippi River. The local business association states that the name Muscatine is not used by any other community.

Muscatine County is a county located in the U.S. state of Iowa. As of the 2020 census, the population was 43,235. The county seat is Muscatine. The southeastern border is formed by the Mississippi River.