Nuclear magnetic resonance spectroscopy
Nuclear magnetic resonance spectroscopy, most commonly known as NMR spectroscopy or magnetic resonance spectroscopy (MRS), is a spectroscopic technique based on re-orientation of atomic nuclei with non-zero nuclear spins in an external magnetic field. This re-orientation occurs with absorption of electromagnetic radiation in the radio frequency region from roughly 4 to 900 MHz, which depends on the isotopic nature of the nucleus and increased proportionally to the strength of the external magnetic field. Notably, the resonance frequency of each NMR-active nucleus depends on its chemical environment. As a result, NMR spectra provide information about individual functional groups present in the sample, as well as about connections between nearby nuclei in the same molecule.
As the NMR spectra are unique or highly characteristic to individual compounds and functional groups, NMR spectroscopy is one of the most important methods to identify molecular structures, particularly of organic compounds.
A 900 MHz NMR instrument with a 21.1 T magnet at HWB-NMR, Birmingham, UK
Cutaway of an NMR magnet that shows its structure: radiation shield, vacuum chamber, liquid nitrogen vessel, liquid helium vessel, and cryogenic shims.
The NMR sample is prepared in a thin-walled glass tube - an NMR tube.
1H NMR spectrum of menthol with chemical shift in ppm on the horizontal axis. Each magnetically inequivalent proton has a characteristic shift, and couplings to other protons appear as splitting of the peaks into multiplets: e.g. peak a, because of the three magnetically equivalent protons in methyl group a, couple to one adjacent proton (e) and thus appears as a doublet.
Spectroscopy is the field of study that measures and interprets electromagnetic spectra. In narrower contexts, spectroscopy is the precise study of color as generalized from visible light to all bands of the electromagnetic spectrum.
An example of spectroscopy: a prism analyses white light by dispersing it into its component colors.
A huge diffraction grating at the heart of the ultra-precise ESPRESSO spectrograph.
UVES is a high-resolution spectrograph on the Very Large Telescope.
Atomic spectra comparison table, from "Spektroskopische Methoden der analytischen Chemie" (1922).