Palestrina is a modern Italian city and comune (municipality) with a population of about 22,000, in Lazio, about 35 kilometres east of Rome. It is connected to the latter by the Via Prenestina. It is built upon the ruins of the ancient city of Praeneste.

Giovanni Pierluigi da Palestrina was an Italian composer of late Renaissance music. The central representative of the Roman School, with Orlande de Lassus and Tomás Luis de Victoria, Palestrina is considered the leading composer of late 16th-century Europe.