Pelican Island National Wildlife Refuge is a United States National Wildlife Refuge (NWR), and part of the Everglades Headwaters NWR complex, located just off the western coast of North Hutchinson Island in the Indian River Lagoon east of Sebastian, Florida. The refuge consists of a 3-acre (12,000 m2) island that includes an additional 2.5 acres (10,000 m2) of surrounding water and is located off the east coast of Florida of the Indian River Lagoon. Established by an executive order of President Theodore Roosevelt on March 14, 1903, Pelican Island was the first National wildlife refuge in the United States. It was created to protect egrets and other birds from extinction through plume hunting. The oldest government wildlife refuge of any kind in North America is the Lake Merritt Bird Refuge in Oakland, California. Oakland Mayor Samuel Merritt declared it a wildlife refuge for migrating birds in 1869. In 1870, the state of California designated Lake Merritt a state game refuge.

National Wildlife Refuge System (NWRS) is a system of protected areas of the United States managed by the United States Fish and Wildlife Service (FWS), an agency within the Department of the Interior. The National Wildlife Refuge System is the system of public lands and waters set aside to conserve America's fish, wildlife, and plants. Since President Theodore Roosevelt designated Florida's Pelican Island National Wildlife Refuge as the first wildlife refuge in 1903, the system has grown to over 568 national wildlife refuges and 38 wetland management districts encompassing about 856,000,000 acres (3,464,109 km2).