The Provincetown Playhouse is a historic theatre at 133 MacDougal Street between West 3rd and 4th streets in the Greenwich Village neighborhood of Manhattan in New York City. It is named for the Provincetown Players, who converted the former stable and wine-bottling plant into a theater in 1918.

The Provincetown Players was a collective of artists, people and writers, intellectuals, and amateur theater enthusiasts. Under the leadership of the husband and wife team of George Cram “Jig” Cook and Susan Glaspell from Iowa, the Players produced two seasons in Provincetown, Massachusetts and six seasons in New York City, between 1916 and 1922. The company's founding has been called "the most important innovative moment in American theatre." Its productions helped launch the careers of Eugene O'Neill and Susan Glaspell, and ushered American theatre into the Modern era.