Roma Trastevere railway station is a major railway station serving the city and comune of Rome, Italy. Opened in 1911, it forms part of the Pisa–Livorno–Rome, Rome–Fiumicino railways (FL1), Rome-Viterbo (FL3) and Rome-Civitavecchia (FL5).

The Pisa–Rome railway is one of the trunk lines of the Italian railway network. It connects Italy’s northwest with its south, running along the Tyrrhenian coast between the Italian regions of Tuscany and Lazio, through the provinces of Livorno, Grosseto, Viterbo and Rome. The line is double track and is fully electrified at 3,000 V DC. Passenger traffic is managed by Trenitalia.