The Spanish conquest of the Muisca took place from 1537 to 1540. The Muisca were the inhabitants of the central Andean highlands of Colombia before the arrival of the Spanish conquistadors. They were organised in a loose confederation of different rulers; the psihipqua of Muyquytá, with his headquarters in Funza, the hoa of Hunza, the iraca of the sacred City of the Sun Sugamuxi, the Tundama of Tundama, and several other independent caciques. The most important rulers at the time of the conquest were psihipqua Tisquesusa, hoa Eucaneme, iraca Sugamuxi and Tundama in the northernmost portion of their territories. The Muisca were organised in small communities of circular enclosures, with a central square where the bohío of the cacique was located. They were called "Salt People" because of their extraction of salt in various locations throughout their territories, mainly in Zipaquirá, Nemocón, and Tausa. For the main part self-sufficient in their well-organised economy, the Muisca traded with the European conquistadors valuable products as gold, tumbaga, and emeralds with their neighbouring indigenous groups. In the Tenza Valley, to the east of the Altiplano Cundiboyacense where the majority of the Muisca lived, they extracted emeralds in Chivor and Somondoco. The economy of the Muisca was rooted in their agriculture with main products maize, yuca, potatoes, and various other cultivations elaborated on elevated fields. Agriculture had started around 3000 BCE on the Altiplano, following the preceramic Herrera Period and a long epoch of hunter-gatherers since the late Pleistocene. The earliest archaeological evidence of inhabitation in Colombia, and one of the oldest in South America, has been found in El Abra, dating to around 12,500 years BP.

The Muisca are an indigenous people and culture of the Altiplano Cundiboyacense, Colombia, that formed the Muisca Confederation before the Spanish conquest. The people spoke Muysccubun, a language of the Chibchan language family, also called Muysca and Mosca. They were encountered by conquistadors dispatched by the Spanish Empire in 1537 at the time of the conquest. Subgroupings of the Muisca were mostly identified by their allegiances to three great rulers: the hoa, centered in Hunza, ruling a territory roughly covering modern southern and northeastern Boyacá and southern Santander; the psihipqua, centered in Muyquytá and encompassing most of modern Cundinamarca, the western Llanos; and the iraca, religious ruler of Suamox and modern northeastern Boyacá and southwestern Santander.