Tampa Bay is a large natural harbor and shallow estuary connected to the Gulf of Mexico on the west-central coast of Florida, comprising Hillsborough Bay, McKay Bay, Old Tampa Bay, Middle Tampa Bay, and Lower Tampa Bay. The largest freshwater inflow into the bay is the Hillsborough River, which flows into Hillsborough Bay in downtown Tampa. Many other smaller rivers and streams also flow into Tampa Bay, resulting in a large watershed area.

A harbor, or harbour, is a sheltered body of water where ships, boats, and barges can be moored. The term harbor is often used interchangeably with port, which is a man-made facility built for loading and unloading vessels and dropping off and picking up passengers. Harbors usually include one or more ports. Alexandria Port in Egypt is an example of a harbor with two ports.