U.S. Route 301 is a part of the U.S. Highway System that runs from Sarasota, Florida, to Biddles Corner, Delaware. In Virginia, the U.S. Highway runs 142.70 miles (229.65 km) from the North Carolina state line near Skippers north to the Maryland state line at the Potomac River near Dahlgren. US 301 forms the local complement to Interstate 95 (I-95) from Emporia to Petersburg. The U.S. Highway runs concurrently with US 1 between Petersburg and the state capital of Richmond, where the highways form one of the main north–south avenues. US 301 continues north concurrent with Virginia State Route 2 to Bowling Green, forming an eastern alternative to I-95 and US 1 north of Richmond. At Bowling Green, which is connected to I-95 by SR 207, US 301 becomes the primary highway connecting Richmond and the Northern Neck with Southern Maryland. US 301 was constructed in four main segments: as the original SR 24 and then US 17-1 south of Petersburg, as US 1 from Petersburg to Richmond, as SR 2 from Richmond to Bowling Green, and as part of SR 207 toward Dahlgren. US 301 replaced US 17–1 in the early 1930s and was extended from Petersburg north along its current course into Maryland when the Potomac River Bridge was completed in 1940.

Interstate 95 (I-95) runs 179 miles (288 km) within the commonwealth of Virginia between its borders with North Carolina and Maryland. I-95 meets the northern terminus of I-85 in Petersburg and is concurrent with I-64 for three miles (4.8 km) in Richmond. Although I-95 was originally planned as a highway through Washington, D.C., it was rerouted along the eastern portion of the Capital Beltway concurrent with I-495. From Petersburg to Richmond, I-95 utilized most of the Richmond–Petersburg Turnpike, a former toll road. In addition to Richmond, the route also runs through the medium-sized cities of Emporia, Petersburg, Colonial Heights, Fredericksburg, and Alexandria.