Founded in 1862, the University of Pennsylvania Glee Club is one of the oldest continually running glee clubs in the United States and the oldest performing arts group at the University of Pennsylvania. The Club draws its singing members from the undergraduate and graduate populations of the University of Pennsylvania; individuals from the Penn community are also called upon to fill roles in the band and technical staff when the Club puts on theatrical productions. The club, known for its eclectic mix of Penn standards, Broadway classics, classical favorites, and pop hits, has traveled to over 40 countries and territories on five continents. After directing the Glee Club for 44 years, Bruce Montgomery stepped down as director in 2000 and was replaced by former Glee Club member C. Erik Nordgren. After 15 years of dedicated service to the group, Nordgren stepped down and was succeeded by Joshua Glassman. After three years at the podium Joshua Glassman stepped down, passing the baton to Club alumnus Daniel Carsello. As of April 9, 2021, the Penn Glee Club accepts singers of all genders.

A glee club in the United States is a musical group or choir group, historically of male voices but also of female or mixed voices, which traditionally specializes in the singing of short songs by trios or quartets. In the late 19th century it was very popular in most schools and was made a tradition to have in American high schools from then on.