Upshur County is a county in the U.S. state of West Virginia. As of the 2020 census, the population was 23,816. Its county seat is Buckhannon. The county was formed in 1851 from Randolph, Barbour, and Lewis counties and named for Abel Parker Upshur, a distinguished statesman and jurist of Virginia. Upshur served as United States Secretary of State and Secretary of the Navy under President John Tyler.

Buckhannon is the only incorporated city in, and the county seat of, Upshur County, West Virginia, United States. Located along the Buckhannon River, the population was 5,299 as of the 2020 census. The city is 46 miles (74 km) southwest of Morgantown, 88 miles (142 km) northeast of the capital city of Charleston, and 100 miles (160 km) south of Pittsburgh, Pennsylvania. It is home to West Virginia Wesleyan College and the West Virginia Strawberry Festival, held annually during the third week of May.